| Summary: | 碩士 === 國立中央大學 === 環境工程研究所 === 83 === The objective of this research was to evaluate the biotreatment
feasibility of phenolic pollutants in water by means of pure
culturewith white-rot fungus Phanerochaete chrysosporium ATCC
24725. The coimmobilizing capsules, alginate-PAC (powdered
activated carbon) gel, were used to immobilize the fungus. The
result haddemonstrated that the coimmobilized-cell system was
more effective than the suspended-cell system in the treatment
of phenolic pollutants including phenol、4-chlorophenol(CP)
、2,4-di- chlorophenol and 2,4,6-trichlorophenol. The growth
of suspended cells had an inverse relation to the dosage of
phenolic compounds . The growthof suspended P. chrysosporium
was inhibited when exposured to a dosage loading with 0.1mg
4-CP/mg-cell, but it seemed have no effect on the growth of
immobilized cells. To identify themetabolites of the phenolic
compounds after degradation by P. chrysosporium, the result
from HPLC analysis indicated that phenol was initially degraded
to catechol, then degraded tomuconic acid, 4-chlorophenol was
oxidized to 4-chloro- catechol,on the other hand,
2,4-dichlorophenol and 2,4,6-tri chlorophenol were
dechlorinated to 4-chlorophenol and phenol, respectively.The
coimmobilization system could retain phenolic compounds in
position near the fungus cells grown on the carriers , thereby
increasing the degradation efficiency for the target chemicals.
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