Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis

• Main Author: 洪欣哲
• Other Authors: 洪良邦
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/49075564906519292426

碩士 === 國立海洋大學 === 水產食品科學研究所 === 83 === 　　Clolesterol and cholest-4-en-3-one were analyzed in packed-column supercritical fluid chromatography (SFC). Cholesterol and cholest-4-en-3-one showed significatantly peak tailing on C8 olumn, but not on CN column. 　　The retention times of cholesterol and cholest-4-en-3-one were related with pressure and temperature. The reteniton time of cholesterol was shorter than cholest-4-en-3-one on CN column, however longer on C8 column. In the range of the column, however longer on C8 column. In the range of the concentraction from 0.2∼20 mg/mL, the correlation coeffecient for the calibration of choleterol in 210 nm was 0.99985, and that of cholest-4-en-3one in 240 nm was 0.99998. 　　On CN column, cholesterol, cholesteryl chloroacetate, cholesteryl phenylacetate, and cholest-4-en-3-one were competely separated by keeping the pressure at 15 MPa and temperature programming from 80℃ to 60℃ at the rate of-3℃ per minute. 　　After improving the hardware of the system, the on-line SFE-cholesterol oxidase-SFC system was found efficient in cholesterol analysis. Cholest-4-en-3-one could be detected in 10 minutes after the cholesterol started reacting with enzyme. The correlation coefficient of the calibration curve was 0.9974.