Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis
碩士 === 國立海洋大學 === 水產食品科學研究所 === 83 === Clolesterol and cholest-4-en-3-one were analyzed in packed-column supercritical fluid chromatography (SFC). Cholesterol and cholest-4-en-3-one showed significatantly peak tailing on C8 olumn, but not on CN column. The retention times of cholesterol and cho...
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ndltd-TW-083NTOU30840272015-10-13T12:26:21Z http://ndltd.ncl.edu.tw/handle/49075564906519292426 Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis 超臨界流體層析法與超臨界流體萃取─膽固醇氧化酉每─超臨界流體層析連法在膽固醇分析上之應用 洪欣哲 碩士 國立海洋大學 水產食品科學研究所 83 Clolesterol and cholest-4-en-3-one were analyzed in packed-column supercritical fluid chromatography (SFC). Cholesterol and cholest-4-en-3-one showed significatantly peak tailing on C8 olumn, but not on CN column. The retention times of cholesterol and cholest-4-en-3-one were related with pressure and temperature. The reteniton time of cholesterol was shorter than cholest-4-en-3-one on CN column, however longer on C8 column. In the range of the column, however longer on C8 column. In the range of the concentraction from 0.2∼20 mg/mL, the correlation coeffecient for the calibration of choleterol in 210 nm was 0.99985, and that of cholest-4-en-3one in 240 nm was 0.99998. On CN column, cholesterol, cholesteryl chloroacetate, cholesteryl phenylacetate, and cholest-4-en-3-one were competely separated by keeping the pressure at 15 MPa and temperature programming from 80℃ to 60℃ at the rate of-3℃ per minute. After improving the hardware of the system, the on-line SFE-cholesterol oxidase-SFC system was found efficient in cholesterol analysis. Cholest-4-en-3-one could be detected in 10 minutes after the cholesterol started reacting with enzyme. The correlation coefficient of the calibration curve was 0.9974. 洪良邦 1995 學位論文 ; thesis 80 zh-TW |
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碩士 === 國立海洋大學 === 水產食品科學研究所 === 83 ===
Clolesterol and cholest-4-en-3-one were analyzed in packed-column supercritical fluid chromatography (SFC). Cholesterol and cholest-4-en-3-one showed significatantly peak tailing on C8 olumn, but not on CN column.
The retention times of cholesterol and cholest-4-en-3-one were related with pressure and temperature. The reteniton time of cholesterol was shorter than cholest-4-en-3-one on CN column, however longer on C8 column. In the range of the column, however longer on C8 column. In the range of the concentraction from 0.2∼20 mg/mL, the correlation coeffecient for the calibration of choleterol in 210 nm was 0.99985, and that of cholest-4-en-3one in 240 nm was 0.99998.
On CN column, cholesterol, cholesteryl chloroacetate, cholesteryl phenylacetate, and cholest-4-en-3-one were competely separated by keeping the pressure at 15 MPa and temperature programming from 80℃ to 60℃ at the rate of-3℃ per minute.
After improving the hardware of the system, the on-line SFE-cholesterol oxidase-SFC system was found efficient in cholesterol analysis. Cholest-4-en-3-one could be detected in 10 minutes after the cholesterol started reacting with enzyme. The correlation coefficient of the calibration curve was 0.9974.
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洪良邦 |
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洪良邦 洪欣哲 |
author |
洪欣哲 |
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洪欣哲 Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis |
author_sort |
洪欣哲 |
title |
Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis |
title_short |
Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis |
title_full |
Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis |
title_fullStr |
Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis |
title_full_unstemmed |
Applications of Supercritical Fluid Chromatography and On-line Supercritical Fluid Extraction-cholesterol Oxidase-supercritical Fluid Chromatography in Cholesterol Analysis |
title_sort |
applications of supercritical fluid chromatography and on-line supercritical fluid extraction-cholesterol oxidase-supercritical fluid chromatography in cholesterol analysis |
publishDate |
1995 |
url |
http://ndltd.ncl.edu.tw/handle/49075564906519292426 |
work_keys_str_mv |
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