A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus
碩士 === 國立臺灣大學 === 解剖學研究所 === 83 === The present study aims to localize the glycine-immunoreactive (glycine-IR) neurons and terminals in the rat cuneate nucleus. Pre-embedding immunostaining was employed to identify the glycine-IR neurons and postembedding imm...
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ndltd-TW-083NTU003910012015-10-13T12:26:22Z http://ndltd.ncl.edu.tw/handle/69641596943437889533 A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus 大白鼠楔狀神經核內甘胺酸性神經元及其與楔狀丘腦接轉神經元突觸關係之研究 Hsieh,Wei-Fang 謝維芳 碩士 國立臺灣大學 解剖學研究所 83 The present study aims to localize the glycine-immunoreactive (glycine-IR) neurons and terminals in the rat cuneate nucleus. Pre-embedding immunostaining was employed to identify the glycine-IR neurons and postembedding immunogold labelling was used to elucidate the glycine-IR terminals in the rat cuneate nucleus. Retrograde transport of wheat germ agglutinin conjugated with horseradish peroxidase complex (WGA-HRP) was use to label CTNs and anti-glycine postembedding immunogold labelling was used for the identification of glycine-IR terminals. Glycine-IR cell bodies were located in the whole rostrocaudal extent of the nucleus and were distributed randomly. The morphology of glycine- IR neuron included round and spindle shape (mean density = 0.86 cell / 10000mm2, SD= 0.32). About 20% of the neurons were glycine-immunoreactive (mean area=167.868.1mm2). At the electron microscopic level, about 24.7% of the terminals (n = 587) surveyed were immunogold-labeled glycine-IR terminals. They were mostly medium size (mean = 1.44 0.7mm2, n= 145) and contained either flatten or pleomorphic vesicles. Most of Glycine-IR terminal formed symmetric synapses with dendrites of various sizes. In addition, glycine-IR terminal also made synaptic contact with axon terminal of unknown origin. The synaptic relation between the glycine-immunoreative (glycine-IR) terminals and cuneothalamic relay neurons (CTNs) in the rat cuneate nucleus were found to form axosomatic, axodendritic and axospinous synapses. With these procedures, immunogold-labelled glycine-IR terminals were medium-sized (mean area= 1.670.7 mm2). Our results suggest that glycine might be one of the important inhibitory neurotransmitters in the cuneate nucleus. Wen,Jenn-Yuan 溫振源 1995 學位論文 ; thesis 78 zh-TW |
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碩士 === 國立臺灣大學 === 解剖學研究所 === 83 === The present study aims to localize the glycine-immunoreactive (glycine-IR) neurons and terminals in the rat cuneate nucleus.
Pre-embedding immunostaining was employed to identify the
glycine-IR neurons and postembedding immunogold labelling was
used to elucidate the glycine-IR terminals in the rat cuneate
nucleus. Retrograde transport of wheat germ agglutinin conjugated with horseradish peroxidase complex (WGA-HRP) was use to label CTNs and anti-glycine postembedding immunogold labelling was used for the identification of glycine-IR terminals. Glycine-IR cell bodies were located in the whole rostrocaudal extent of the nucleus and were distributed randomly. The morphology of glycine- IR neuron included round and spindle shape (mean density = 0.86 cell / 10000mm2, SD= 0.32). About 20% of the neurons were glycine-immunoreactive (mean area=167.868.1mm2). At the electron microscopic level, about 24.7% of the terminals (n = 587) surveyed were immunogold-labeled glycine-IR terminals. They were mostly medium size (mean = 1.44 0.7mm2, n= 145) and contained either flatten or pleomorphic vesicles. Most of Glycine-IR terminal formed symmetric synapses with dendrites of various sizes. In addition, glycine-IR terminal also made synaptic contact with axon terminal of unknown origin. The synaptic relation between the glycine-immunoreative (glycine-IR) terminals and cuneothalamic relay neurons (CTNs) in the rat cuneate nucleus
were found to form axosomatic, axodendritic and axospinous
synapses. With these procedures, immunogold-labelled glycine-IR
terminals were medium-sized (mean area= 1.670.7 mm2). Our
results suggest that glycine might be one of the important
inhibitory neurotransmitters in the cuneate nucleus.
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author2 |
Wen,Jenn-Yuan |
author_facet |
Wen,Jenn-Yuan Hsieh,Wei-Fang 謝維芳 |
author |
Hsieh,Wei-Fang 謝維芳 |
spellingShingle |
Hsieh,Wei-Fang 謝維芳 A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
author_sort |
Hsieh,Wei-Fang |
title |
A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
title_short |
A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
title_full |
A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
title_fullStr |
A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
title_full_unstemmed |
A study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
title_sort |
study on the synaptic relation between glycine-immunoreactive neurons and cuneothalamic relay neurons in the rat cuneate nucleus |
publishDate |
1995 |
url |
http://ndltd.ncl.edu.tw/handle/69641596943437889533 |
work_keys_str_mv |
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