Reproductive periodicity and larval culture of two sea urchins anthocidaris crassispina and stomopneustes variolaris

• Main Authors: Lin, Shun Kang, 林順康
• Other Authors: Chen, Zhang Po
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/59839164006548401209

碩士 === 國立臺灣大學 === 漁業科學研究所 === 83 === Reproduction periodicities of Anthocidaris crassispina and Stomopneustes variolaris, around the coastal of Yeh-liu in northern Taiwan(25.1。N), were studies by compared with monthly gonad index and histological examination of gonads from Mar. to Dec. 1994. The spawning period of A. crassispina was from Jul. to Sep., between the populations of Japan (32.8。N, Jul.- Aug.) and Hong Kong (22.2。N, Jun.-Oct.). The spawning season of S. variolaris was from Jun. to Oct., longer than South African ( 30.5。S, Dec.-Jan.). Spawning of both sea urchins was initiated by injection of 0.5M KCI solution. Eggs were rinsed with filt- ered seawater, fertilization with a dilute suspension of sperm, and rinsed several times with filtered seawater (35 ppt S). After hatching, the swimming, pyramidal-shaped larvae were transferred to 2L beakers filled with 1.5L of filtered seawater. The seawater in the culture beakers were continuously rotated ( 10 rpm ) at 25 ℃ in a dark room and renewed every day. After changed the seawater, the microalgae Isochrysis galbana was used as food for larvae at concentrations of 104 cells / ml. After 6 -arms stage, larvae were processed by feeding different alga species, I. galbana, Tetraselmis chui and Skeletonema costatum, at different concentrations of 104, 5*104 and 105 cells/ml. The larvae of A. crassispina, fed on I. galbana, grew best, and better at higher algal concentration ( 105 cells/ml). In contrast, the S. variolaris larvae had the best growth at lower algal concentration ( 104 cells / ml ) and no significant difference between different algal treatments. The larvae of A. crassispina, fed with 105 cells / ml of I. galbana at 24 ℃, reached the 8-arms stage 12d after fertilization. The ruidiment and 2 pedicellariae appeared at 19 d. The larvae of S. variolaris,reared at 104 cells/ml of I. galbana at 25 ℃, reached the 12-arms at 37d. The larvae with ruidiment were found at 39d.