Studies on the Active Components of Gardeniae Fructus, Paeoniae Radix, and Xanthii Fructus

• Main Author: 黃慕淇
• Other Authors: 徐鳳麟
• Format: Others
• Language: zh-TW
• Published: 1995
• Online Access: http://ndltd.ncl.edu.tw/handle/39569129156647228032

碩士 === 台北醫學大學 === 藥學研究所 === 83 === In order to clarify the active components of Chinese herbs frequently used to treat diabetes mellitus in Taiwan, Gardeniae Fructus, Paeoniae Radix, and Xanthii Fructus were chosen for furthur investigation based on their positive preliminary results. We isolated 14 compounds from above three mentioned plants by using various column chromatography including MPLC and HPLC. Their structures were determined based on the analysis of NMR, MS spectra and X-ray single crystal diffraction. Six compounds obtained from Gardeniae Fructus were genipin, geniposide, genipin -1-β-D- gentiobioside, shanzhiside, gardenoside and methyideacetylasperulosidate. Three compounds isolated from Paeoniae Radix were paeoniflorin, 8-debenzoylpaeoniflorin and β-sitosterol-3-β-D-glucoside. Five compounds obtained from Xanthii Fructus were caffeic acid, potassium 3-O-caffeoyl quinate, 1,5-di-O-caffeoyl quinic acid, 1,3,5-tri-O-caffeoyl quinic acid and 7-hydroxymethyl-8,8-dimethyl-4,8-dihydro-benzo[1,4]thiazine-3,5-dione. Among them, paeoniflorin, 8-debenzoylpaeoniflorin and potassium 3-O-caffeoyl quinate, 1,5-di-O-caffeoyl quinic acid, 1,3,5-tri-O-caffeoyl quinic acid and 7-hydroxymethyl-8,8-dimethyl-4,8-dihydro-beiizo[1,4]thiazine-3,5-dione were found to possess hypoglycemic activity. The 7-hydroxymethyl-8,8-dimethyl-4,8-dihydro-benzo[1,4]tluazine-3,5-dione is a new compound.