| Summary: | 碩士 === 中山醫學院 === 生物化學研究所 === 84 === The prevalence of asthma in scool-age children in Taipei
increased from 1.3% to 10.8% during the past twenty years .It is
found that HDM (house dust mite ) is tje most important
allergens in Taiwan ,especially for asthmatic children.this
study is divided into two parts.In the first part the author
used the affinity puriffication of human sera that attaches
specific Der p5 antibodies withthe Der p5 recombinant fusion
protein .The result will elute antibody in mite extraction and
rDer p5 fusion protein to corresponding to components of Mr 14kd
and 42 kd that can identify the natural allergen -Der p5 .The
samplesare drawn from the 12 families in Taichung .Inclusion
criteria:the family must has a child attacked asthma .The dust
samples were collected from the mattress and the floors of thee
bedroom and living room of the children homeenviroment from Oct
1994 to Sep 1995 .In the research finding ,the highest
concentration of dust samples ranges from 6-8000 ng/g fine dust
,The highest samples were oberved the mattress .The lowest
concentration in the floors of the bedroom.In the second part,
the study is focused on the reaction frequency and relationsship
betwwen antibodies(IgG and IgE)and purified mite allergen (Der
p5) in different groups ,according to the result ,the Der p5 has
the significance in the clinical finding .THe antibody (IgE) in
the serum has the highly affinity and frequency in the children
of asthma
|
|---|
