| Summary: | 碩士 === 高雄醫學院 === 天然藥物研究所 === 84 === Abstract "Gou-Qi" is one of the most popular nutrients and
traditional medicines in China and Japan . It is derived from
the following plants of the Solanaceae family: (a) Lycium
chinense MILL. (b) L. barbarum L.[=L. halimifolium MILL.]. The
plant is a good treatment for trouble withlung, blood, bones,
and kidney. The root, aerial part, and fruit areused as a remedy
to chronic illnesses, increase the vision, invigorating for
liver and kidney. And further, the plant was also be used as
antirheumatic, diuretic, antisenile agent and antipyretic for
hepatitis. However, the mechanism is obscure. In recent years,
it has been reported that free radicals intermediates such as
.O2- (superoxide anion radical), .OH (hydroxyl radical), H2O2
(hydrogen peroxide) and 1O2 ( singalet oxygen) are notonly one
of important class of cellular injuries but also can initiate
peroxidation of polyunsaturated fatty acids in biological
membranes. Thelipid peroxidation can lead to diverse
pathological conditions,including various liver disorders,
atherosclerosis, rheumatic diseases,irradiation and aging by
injuring the liver, kidney and other organs. In the other hand,
the superoxide scavenger such as superoxide dismutase(SOD) and
vitamin E are capable of alleviating free radical damage.
Based on these findings, some of the pharmacological mechanism
of this plant may be come form it''s SOD-like activity or
eliminates the production of excess active oxygen and lipid
peroxide. In this study, it was interested to evaluated the
relationship between pharmacological and antioxidant effect of
this plant. The carrageenan induced rat paw edema and CCl4
induced hepatotoxicity inrats were used to study the anti-
inflammatory and liver protective effects of L. chinense. The
histological changes of rat hepatic tissues were also
investigated. Furthermore, the antioxidant effect of the crude
drug extract are evaluated by two experiments: FeCl2-Ascorbic
acid induced lipid peroxidation in rat liver homogenate and
active oxygen scavenging activity by electron spin resonance
(ESR) spectrometry using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO)
as a spin trap. The extracts of L. chinense show anti-
inflammatory activity against carragenan induced rats paw edema
and CCl4-induced liver injury. Results also exhibited anti-
oxidant effects in FeCl2-ascorbate induced lipid peroxidation in
rat liver homogenate. Moreover, the free radical scavenger
effect of L. chinense was demonstrated using electron spin
resonance and spin-trapping technique. Based on these findings,
the pharmacological active of L. chinense possibly involves
mechanisms related to free radical scavenger activity.
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