| Summary: | 碩士 === 國立中興大學 === 植物病理學系 === 84 === Sweet pepper plants grown in the fields with leaf showing
symptoms of crinkle and mottle were collected as the studing
material. Three isolates ( L1, L2 and L3 ) were isolated with
different local lesion indicator. Identification of the three
virus isolates was further conducted by host reactions, physical
properties, serological tests and electron microscopy. All of
these three virus isolates had the same characteristics. They
caused yellow ring spots on Chenopodium amaranticolor, C.
quinoa, Datura metel and same system symptoms on other indicator
plants, their thermal incativation point were 60~70℃, dilution
end point were 10-6~10-7 , and the particles were all similar as
540~560 nm × 13nm. According to the same properties, these
three isolates from Taiwan were conduced as the same strain of
potato virus X. The titers of the antisera produced from rabbit
by injecting purified virus as antigen was 1024 as determined by
the interface precipitin test. Two different enzymes ( alkaline
phosphatase and horse-radish peroxidase ) were conjugated to the
fractionated antibody for the use in DAS-ELISA ( double antibody
sandwich immunosorbent assay ) to compare the efficiency on
detecting virus. When the peroxidase inhibitor such as NaN3 or
H2O2 in ethanol was added to the plant crude extract with 30 min
before loading the enzyme conjugated antibody in DAS-ELISA
processing, the background reaction was reduced. HRP-antibody
conjugated by periodate-oxidation precisely detected the virus
at the concentration of 1: 2000 and the reations were easily
visulized after the treatment with adding peroxidase inhibitors
such as NaN3 and H2O2 in ethonal before loading the HRP
conjugate antibody.
|
|---|
