| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 84 === In most photosynthetic and chemoautotrophic organisms, CO2 is
fixed into organic carbon by means of the reductive pentose
phosphate pathway. Transketolase is the key enzyme of the non-
oxidative part of the pentose phosphate pathway. Oleosin are a
group of Mr 15~26 kD amphipathic proteins which are associated
with the surface of oil bodies in lipid-storing tissues such as
seeds and pollen. The role of oleosin in oil body is biogenesis
and structural stabilization. This research focuses on the use
of cabbage (Brassica oleracea L. var. capitata L.) and Chinese
cabbage (Brassica campestris L. ssp. pekinensis) as a model
system to establish the gene transfer technology, and
possibility for improvement of cabbage and Chinese cabbage with
stress resistance and nutrient quality, through the art of
genetic engineering. Transketolase (TKL) and Oleosin (OLE) genes
were isolated from yeast and rice, respectively, constructed
into plant transformation vectors drived by CaMV 35S or rubisco
small subunit (rbc S) promoter, and introduced into the
hypocotyl and cotyledon of cabbage and Chinese cabbage using
Agrobacterium-mediated transformation. Regenerated plants of
cabbage and Chinese cabbage were obtained after transformation
with six kinds of plasmids. The regeneration rates from cabbage
and Chinese cabbage hypocotyl were 2.3% ~ 9.0% and 0.2 ~ 2%
respectively. Only few plants were regenerated from the
cotyledon of cabbage and Chinese cabbage. The regenerated plants
were examined by PCR, southern, and Northern hybridization.The
results indicated that the expression of constructed genes was
higher in transgenic plants drived by the rbc S promoter than by
CaMV 35S promoter. The TKL-transformed "New Top" cabbage had
higher TKL activity and heat resistance than the controls.
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