| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 84 === AbstractEnvironmental factors are the most important matters
affecting the growth and distribution of the crops and in which
temperature is especial importance. Low temperature affects a
wide range of plant species when they are subjected to low
temperature stress, and also causes plant loss and reduced
yield. Heavy metal pollution is the major problem that has been
discussed in a few years. High heavy metal concentration in soil
will affect the growth and product of crops, and the health of
human body. The purposes of this research are to establish the
gene transfer technology of cruciferous vegetables, to study the
effects of promoters on cadmium-binding protein gene (MT II) and
antifreezing protein gene (AF) expression and to study the
possibility for improvement of cruciferous vegetable with heavy
metal and frost resistance, through the art of genetic
engineering.We had constructed the MT II of Chinese hamster and
AF of winter flounder with the combinations of CaMV 35S or
rubisco small subunit (rbcS) promoters as plant transfer
vectors. The constructed plasmids were transferred into
hypocotyl and cotyledon of broccoli (Green King), cabbage (K-Y
cross and Tropical Delight), and Chinese cabbage (Tropical
pride) via Agrobacterium mediated transformation.Regenerated
plants of Brassica vegetables were obtained after transformation
with twelve kinds of plasmids. The regeneration rates from
broccoli, cabbage, and Chinese cabbage hypocotyl were 11.0 % ~
4.0 %, 1.1 % ~ 0.11 %, and below 0.48 % , respectively. None was
regenerated from cotyledon of three Brassica vegetables. The
regenerated plants were examined by PCR, southern and Northern
bolt hybridization. The results indicated that the expression of
constructed genes was higher in transgenic plants drived byone
or two of the rbcS promoter than by CaMV 35S promoter.
Increasing in freezing tolerance and Cd resistance were founded
in AF- and MT-II transformed plants.
|
|---|
