Effects of promoters on the expression of antifreeze protein and Cd-binding protein genes in the transformed broccoli, cabbage, and Chinese cabbage.

• Main Authors: Chen, Shu-Hui, 陳淑惠
• Other Authors: Menq-Jiau Tseng
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/60427750446597621590

碩士 === 國立中興大學 === 園藝學系 === 84 === AbstractEnvironmental factors are the most important matters affecting the growth and distribution of the crops and in which temperature is especial importance. Low temperature affects a wide range of plant species when they are subjected to low temperature stress, and also causes plant loss and reduced yield. Heavy metal pollution is the major problem that has been discussed in a few years. High heavy metal concentration in soil will affect the growth and product of crops, and the health of human body. The purposes of this research are to establish the gene transfer technology of cruciferous vegetables, to study the effects of promoters on cadmium-binding protein gene (MT II) and antifreezing protein gene (AF) expression and to study the possibility for improvement of cruciferous vegetable with heavy metal and frost resistance, through the art of genetic engineering.We had constructed the MT II of Chinese hamster and AF of winter flounder with the combinations of CaMV 35S or rubisco small subunit (rbcS) promoters as plant transfer vectors. The constructed plasmids were transferred into hypocotyl and cotyledon of broccoli (Green King), cabbage (K-Y cross and Tropical Delight), and Chinese cabbage (Tropical pride) via Agrobacterium mediated transformation.Regenerated plants of Brassica vegetables were obtained after transformation with twelve kinds of plasmids. The regeneration rates from broccoli, cabbage, and Chinese cabbage hypocotyl were 11.0 % ~ 4.0 %, 1.1 % ~ 0.11 %, and below 0.48 % , respectively. None was regenerated from cotyledon of three Brassica vegetables. The regenerated plants were examined by PCR, southern and Northern bolt hybridization. The results indicated that the expression of constructed genes was higher in transgenic plants drived byone or two of the rbcS promoter than by CaMV 35S promoter. Increasing in freezing tolerance and Cd resistance were founded in AF- and MT-II transformed plants.