PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN
碩士 === 國立屏東技術學院 === 環境工程技術研究所 === 84 === This study was conducted to isolate biosurfactant producing microorganismsfrom contaminated soil and wastewater in southern Taiwan area. Two Bacillusspecies, NPPI-LP-2 and NPPI-LG-9 were isolated from soil an...
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ndltd-TW-084NPUST5140022015-10-13T14:34:58Z http://ndltd.ncl.edu.tw/handle/89758457419455410926 PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN 本土性篩選之Bacillussp.NPPI-LP-2與BacillussubtilisNPPI-LG-9生物界面活性劑之生產及其特性探討 Hu, Yao-Huang 胡耀湟 碩士 國立屏東技術學院 環境工程技術研究所 84 This study was conducted to isolate biosurfactant producing microorganismsfrom contaminated soil and wastewater in southern Taiwan area. Two Bacillusspecies, NPPI-LP-2 and NPPI-LG-9 were isolated from soil and showed good emul-sifying activity against kerosene. When Bacillus sp. NPPI-LP-2 was cultivatedunder optimal conditions (pH 7.0, 30℃, 150rpm) and optimal medium composition(20g glucose, 2.0g CH3COONH4,1.0g K2 HPO4, 1.0g yeast extract, 0.2g MgSO4.7H20,0.001g FeSO4.7H2O, 0.001g MnSO4.4H2O in one liter of medium ), 1.2g/L of abiosurfactant was produced. The crude biosurfactant could be precipitated byammonium sulfate with chemical composition of carbohydrate (21.9%), protein(16.5%),and lipid (11.1%),mannose was defected by HPLC after being hydrolysed,indicating that it might be a mannolipoprotein. The biosurfactant was heatstable and produced an effective and stable oil-in-water emulsion over a widepH range (5~11). The critical micelle concentration (CMC) of biosurfactant was500mg/L and lowered the surface tension of water from 72mN/m to 39mN/m. Themedian lethal concentration ( LC50 ) for Gambusia affinis after 24 hourstreatment was 850mg/L. The results also indicated that the biosurfactant wasbiodegradable. The optimal medium composition for production of biosurfactant by Bacillussubtilis NPPI-LG-9 was (per liter) : glucose 20.0g, NaNO3 4.0g , K2HPO4 1.5g,yeast extract 0.5g, MgSO4.7H2O 0.05g, KH2PO41.0g,FeSO4.7H2O 0.001g, ZnSO4.7H2O0.001g, MnSO4.4H2O 0.001g. Maximum emulsifying activity was obtained when thebacterium was cultured under optimal conditions (pH 7.0, 30℃, 150rpm) with abiosurfactant yield of 0.9g/L. The crude biosurfactant which was precipitatedby ammonium sulfate then extract by methanol , contained 5.6% carbohydrate,35.6%protein and 48.9% lipid. The CMC of this biosurfactant was 100mg/L ,it could lower the surface tension of water from 72mN/m to 32mN/m . The LC50for Gambusia affinis after 24 hours treatment was 45mg/L. The heat stablebiosurfactant produced by NPPI-LG-9 was biodegradable and could enhance thedegradation of crude oil. Key words : Bacillus, biosurfactant, screen, culture condition, recovery,characteristics. creen, culture condition, recovery, characteristics. Wen-Ing Hwang 黃文瑛 1996 學位論文 ; thesis 122 zh-TW |
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碩士 === 國立屏東技術學院 === 環境工程技術研究所 === 84 === This study was conducted to isolate biosurfactant producing
microorganismsfrom contaminated soil and wastewater in southern
Taiwan area. Two Bacillusspecies, NPPI-LP-2 and NPPI-LG-9
were isolated from soil and showed good emul-sifying activity
against kerosene. When Bacillus sp. NPPI-LP-2 was
cultivatedunder optimal conditions (pH 7.0, 30℃, 150rpm) and
optimal medium composition(20g glucose, 2.0g CH3COONH4,1.0g K2
HPO4, 1.0g yeast extract, 0.2g MgSO4.7H20,0.001g FeSO4.7H2O,
0.001g MnSO4.4H2O in one liter of medium ), 1.2g/L of
abiosurfactant was produced. The crude biosurfactant could be
precipitated byammonium sulfate with chemical composition of
carbohydrate (21.9%), protein(16.5%),and lipid (11.1%),mannose
was defected by HPLC after being hydrolysed,indicating that it
might be a mannolipoprotein. The biosurfactant was
heatstable and produced an effective and stable oil-in-water
emulsion over a widepH range (5~11). The critical micelle
concentration (CMC) of biosurfactant was500mg/L and lowered the
surface tension of water from 72mN/m to 39mN/m. Themedian
lethal concentration ( LC50 ) for Gambusia affinis after 24
hourstreatment was 850mg/L. The results also indicated that
the biosurfactant wasbiodegradable.
The optimal medium composition for production of biosurfactant
by Bacillussubtilis NPPI-LG-9 was (per liter) : glucose 20.0g,
NaNO3 4.0g , K2HPO4 1.5g,yeast extract 0.5g, MgSO4.7H2O 0.05g,
KH2PO41.0g,FeSO4.7H2O 0.001g, ZnSO4.7H2O0.001g, MnSO4.4H2O
0.001g. Maximum emulsifying activity was obtained when
thebacterium was cultured under optimal conditions (pH 7.0,
30℃, 150rpm) with abiosurfactant yield of 0.9g/L. The crude
biosurfactant which was precipitatedby ammonium sulfate then
extract by methanol , contained 5.6% carbohydrate,35.6%protein
and 48.9% lipid. The CMC of this biosurfactant was 100mg/L
,it could lower the surface tension of water from 72mN/m to
32mN/m . The LC50for Gambusia affinis after 24 hours
treatment was 45mg/L. The heat stablebiosurfactant produced by
NPPI-LG-9 was biodegradable and could enhance thedegradation
of crude oil.
Key words : Bacillus, biosurfactant, screen, culture
condition, recovery,characteristics.
creen, culture condition, recovery,
characteristics.
|
author2 |
Wen-Ing Hwang |
author_facet |
Wen-Ing Hwang Hu, Yao-Huang 胡耀湟 |
author |
Hu, Yao-Huang 胡耀湟 |
spellingShingle |
Hu, Yao-Huang 胡耀湟 PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN |
author_sort |
Hu, Yao-Huang |
title |
PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN |
title_short |
PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN |
title_full |
PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN |
title_fullStr |
PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN |
title_full_unstemmed |
PRODUCTION AND CHARACTERISTICS OF BIOSUSFACTANTS BY BACILLUS SP. NPPI-LP-2 AND BACILLUS SUBTILIS NPPI-LG-9 SCREENED FROM TAIWAN |
title_sort |
production and characteristics of biosusfactants by bacillus sp. nppi-lp-2 and bacillus subtilis nppi-lg-9 screened from taiwan |
publishDate |
1996 |
url |
http://ndltd.ncl.edu.tw/handle/89758457419455410926 |
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