Kinetic Study of Aminopeptidase Catalyzed Hydrolysis of Ester and Amide Substrates
碩士 === 國立臺灣大學 === 化學學系 === 84 === Aminopeptidase-I(AP-I)為市售Pronase E所分離純化出的一種外胜酵 素。在含鈣與不含鈣條件中,對於酯類基質的親和性依次為His>Trp>> Phe> Leu>Val>Ala>Gly>>Lys>Arg。催化速率則以Leu最好,Phe次之,其 餘皆無法被AP-I水解。羧基端的烴基團愈大時會因立體阻礙與推電子效應 而使親和性與催...
Main Authors: | Ding, Yi-Chuan, 丁一權 |
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Other Authors: | Lin, Wann-Yin |
Format: | Others |
Language: | zh-TW |
Published: |
1996
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Online Access: | http://ndltd.ncl.edu.tw/handle/29665447988363150115 |
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