Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan

碩士 === 國立臺灣大學 === 獸醫學系 === 84 === In order to differentiate the infectious bronchitis virus (IBV) isolates in Taiwan, polymerase chain reaction and restric- tion fragment length polymorphism (PCR-RFLP) were used to type 12 Taiwan isolates of IBV. Two sets...

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Main Authors: Chang, Pan-Chung, 張泮崇
Other Authors: Wang, Ching-Ho
Format: Others
Language:zh-TW
Published: 1996
Online Access:http://ndltd.ncl.edu.tw/handle/57079548910296951355
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spelling ndltd-TW-084NTU005410142016-07-13T04:10:54Z http://ndltd.ncl.edu.tw/handle/57079548910296951355 Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan 傳染性支氣管炎病毒台灣分離株S1醣蛋白基因之分型及定序 Chang, Pan-Chung 張泮崇 碩士 國立臺灣大學 獸醫學系 84 In order to differentiate the infectious bronchitis virus (IBV) isolates in Taiwan, polymerase chain reaction and restric- tion fragment length polymorphism (PCR-RFLP) were used to type 12 Taiwan isolates of IBV. Two sets of primers were used in this study to amplify the whole length of S1 glycoprotein gene. PCR products were digested with three restriction enzymes, i.e., HaeIII, XcmI and BstYI. Four RFLP patterns were obtained and they were different from those of all foreign IBV strains; the foreign strains are divided into 4 different genetic groups, i. e., Dutch, American, European and Massachusetts. Two IBV Taiwan isolates were selected for S1 gene cloning and sequencing. The nucleotide sequences of these two isolates have 94% similiarity, and those of the deduced amino acid sequence are 90%. The two isolates are close to the Massachusetts group by phylogenetic analysis. The amino acid sequences of the proteolytic cleavage site between the S1 and S2 glycoproteins of the two isolates are both RRFRR, and it is the same with the Massachusetts group. Four consensus-like sequence, CTT(A/T)(A/T) G, are noted in both of the two isolates but no recombination are found. Wang, Ching-Ho 王金和 1996 學位論文 ; thesis 101 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立臺灣大學 === 獸醫學系 === 84 === In order to differentiate the infectious bronchitis virus (IBV) isolates in Taiwan, polymerase chain reaction and restric- tion fragment length polymorphism (PCR-RFLP) were used to type 12 Taiwan isolates of IBV. Two sets of primers were used in this study to amplify the whole length of S1 glycoprotein gene. PCR products were digested with three restriction enzymes, i.e., HaeIII, XcmI and BstYI. Four RFLP patterns were obtained and they were different from those of all foreign IBV strains; the foreign strains are divided into 4 different genetic groups, i. e., Dutch, American, European and Massachusetts. Two IBV Taiwan isolates were selected for S1 gene cloning and sequencing. The nucleotide sequences of these two isolates have 94% similiarity, and those of the deduced amino acid sequence are 90%. The two isolates are close to the Massachusetts group by phylogenetic analysis. The amino acid sequences of the proteolytic cleavage site between the S1 and S2 glycoproteins of the two isolates are both RRFRR, and it is the same with the Massachusetts group. Four consensus-like sequence, CTT(A/T)(A/T) G, are noted in both of the two isolates but no recombination are found.
author2 Wang, Ching-Ho
author_facet Wang, Ching-Ho
Chang, Pan-Chung
張泮崇
author Chang, Pan-Chung
張泮崇
spellingShingle Chang, Pan-Chung
張泮崇
Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan
author_sort Chang, Pan-Chung
title Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan
title_short Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan
title_full Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan
title_fullStr Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan
title_full_unstemmed Genotype Determination and Sequencing of S1 Glycoprotein Genes of Infectious Bronchitis Viruses in Taiwan
title_sort genotype determination and sequencing of s1 glycoprotein genes of infectious bronchitis viruses in taiwan
publishDate 1996
url http://ndltd.ncl.edu.tw/handle/57079548910296951355
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