Cellular factors involved in the replication of hepatitis delta virus

碩士 === 國立臺灣大學 === 生化學研究所 === 84 === HDV-encoded proteins, HDAg-S and HDAg-L of delta antigen, are nuclear phosphoproteins. The HDAg-S appears to be required for the replication of HDV replication and is required for the assembly of HDV....

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Main Authors: Zhang, Min Li, 張旻立
Other Authors: Zhang, Ming Fu
Format: Others
Language:zh-TW
Published: 1996
Online Access:http://ndltd.ncl.edu.tw/handle/40911215295475958851
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spelling ndltd-TW-084NTU021040072016-07-13T04:10:55Z http://ndltd.ncl.edu.tw/handle/40911215295475958851 Cellular factors involved in the replication of hepatitis delta virus 細胞因子參與D型肝炎病毒之複製 Zhang, Min Li 張旻立 碩士 國立臺灣大學 生化學研究所 84 HDV-encoded proteins, HDAg-S and HDAg-L of delta antigen, are nuclear phosphoproteins. The HDAg-S appears to be required for the replication of HDV replication and is required for the assembly of HDV. Cellular factors may be involved in controlling the process of HDV replication. In this study, whether the retinoblastoma protein (RB) and p53 can interact with HDAg-S and affect HDV replication is examined. Coimmunoprecipitation study with rabbit polyclonoal antibodies specifically against the C terminus of the human RB demonstrate an interaction between the HDAg-S and RB. However, the HDAg-S could not be coimmunoprecipitation with p53 in the presence of a monoclonal antibody against p53. By transfecting a dimeric HDV cDNA together with a plasmid encoding RB and p53 into Saos-2 cells, RB was demonstrated to transactivate the replication of HDV, whereas p53 appears to inhibit the replication of HDV. It is the effect of p53 on the replicaiton of HDV because Saos-2 cells transfected with p53 may induce apoptosis when the normal function of RB is lacking. It has been demostrated that Saos-2 cells transfected with RB were arrested in the late G1 phase. This may provide abundant free transcriptional machinery of the transfected Saos-2 cells to support the replication of HDV. In addition, the formation of RB-HDAg-S complexes may induce cytopathic effects in HDV-infected cells. Zhang, Ming Fu 張明富 1996 學位論文 ; thesis 80 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立臺灣大學 === 生化學研究所 === 84 === HDV-encoded proteins, HDAg-S and HDAg-L of delta antigen, are nuclear phosphoproteins. The HDAg-S appears to be required for the replication of HDV replication and is required for the assembly of HDV. Cellular factors may be involved in controlling the process of HDV replication. In this study, whether the retinoblastoma protein (RB) and p53 can interact with HDAg-S and affect HDV replication is examined. Coimmunoprecipitation study with rabbit polyclonoal antibodies specifically against the C terminus of the human RB demonstrate an interaction between the HDAg-S and RB. However, the HDAg-S could not be coimmunoprecipitation with p53 in the presence of a monoclonal antibody against p53. By transfecting a dimeric HDV cDNA together with a plasmid encoding RB and p53 into Saos-2 cells, RB was demonstrated to transactivate the replication of HDV, whereas p53 appears to inhibit the replication of HDV. It is the effect of p53 on the replicaiton of HDV because Saos-2 cells transfected with p53 may induce apoptosis when the normal function of RB is lacking. It has been demostrated that Saos-2 cells transfected with RB were arrested in the late G1 phase. This may provide abundant free transcriptional machinery of the transfected Saos-2 cells to support the replication of HDV. In addition, the formation of RB-HDAg-S complexes may induce cytopathic effects in HDV-infected cells.
author2 Zhang, Ming Fu
author_facet Zhang, Ming Fu
Zhang, Min Li
張旻立
author Zhang, Min Li
張旻立
spellingShingle Zhang, Min Li
張旻立
Cellular factors involved in the replication of hepatitis delta virus
author_sort Zhang, Min Li
title Cellular factors involved in the replication of hepatitis delta virus
title_short Cellular factors involved in the replication of hepatitis delta virus
title_full Cellular factors involved in the replication of hepatitis delta virus
title_fullStr Cellular factors involved in the replication of hepatitis delta virus
title_full_unstemmed Cellular factors involved in the replication of hepatitis delta virus
title_sort cellular factors involved in the replication of hepatitis delta virus
publishDate 1996
url http://ndltd.ncl.edu.tw/handle/40911215295475958851
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