Cellular factors involved in the replication of hepatitis delta virus

• Main Authors: Zhang, Min Li, 張旻立
• Other Authors: Zhang, Ming Fu
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/40911215295475958851

碩士 === 國立臺灣大學 === 生化學研究所 === 84 === HDV-encoded proteins, HDAg-S and HDAg-L of delta antigen, are nuclear phosphoproteins. The HDAg-S appears to be required for the replication of HDV replication and is required for the assembly of HDV. Cellular factors may be involved in controlling the process of HDV replication. In this study, whether the retinoblastoma protein (RB) and p53 can interact with HDAg-S and affect HDV replication is examined. Coimmunoprecipitation study with rabbit polyclonoal antibodies specifically against the C terminus of the human RB demonstrate an interaction between the HDAg-S and RB. However, the HDAg-S could not be coimmunoprecipitation with p53 in the presence of a monoclonal antibody against p53. By transfecting a dimeric HDV cDNA together with a plasmid encoding RB and p53 into Saos-2 cells, RB was demonstrated to transactivate the replication of HDV, whereas p53 appears to inhibit the replication of HDV. It is the effect of p53 on the replicaiton of HDV because Saos-2 cells transfected with p53 may induce apoptosis when the normal function of RB is lacking. It has been demostrated that Saos-2 cells transfected with RB were arrested in the late G1 phase. This may provide abundant free transcriptional machinery of the transfected Saos-2 cells to support the replication of HDV. In addition, the formation of RB-HDAg-S complexes may induce cytopathic effects in HDV-infected cells.