Production of bacterial cellulose by acetobacter aceti subspecies xylinum from citrus juice

碩士 === 國立臺灣大學 === 園藝學研究所 === 84 === Nata is a fine cellulose pellicle formed by the bacteria of Acetobacter aceti subsp. xylinum through the fermentation of fruit juice or plant extract. The objectives of this study are t...

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Bibliographic Details
Main Authors: He, Jia Lun, 何佳倫
Other Authors: Xu, Ming Ren
Format: Others
Language:zh-TW
Published: 1996
Online Access:http://ndltd.ncl.edu.tw/handle/48056479854842933314
Description
Summary:碩士 === 國立臺灣大學 === 園藝學研究所 === 84 === Nata is a fine cellulose pellicle formed by the bacteria of Acetobacter aceti subsp. xylinum through the fermentation of fruit juice or plant extract. The objectives of this study are to investigate the effect of fermentation conditions to the nata formation using Ponkan citrus juice, Valencia orange juice, and their pulp-wash juices as substrates, and to examine the physico-chemical properties of the nata formed under different fermentation conditions. Fermentation of juice samples using a two-stage method resulted in better yields as compared to non-shaking control experiments. The nata formed by the fermentation of single strength juices had better yields as compared to nata formed by using pulp-wash juice as substrate. For pulp-wash juices containing low soluble solid, it is necessary to fortified the juices with sugar to approximately 10°Brix. Pulp-wash juices fortified with fructose gives the highest nata yield as compared to glucose and sucrose. There is no significant difference on nata yield among using coconut milk and respective citrus juices in the first stage fermentation then using Ponkan juice or Valencia juice as substrate in the second stage. However, averaged nata yield of 0.28g/mL is obtained when using coconut milk as first stage substrate and Valencia pulp-wash juice as second stage substrate, which is higher than the averaged yield of 0.14-0.20g/mL in fermentation processes using Valencia pulp-wash juice as substrate at both first and second stages. The nata''s lower interface colors varies depending on the types of citrus juice as substrates. The results of texture analysis indicates that nata from citrus juice fermentation has similar texture to the nata de coco.