Development of Cobalt(II)hexacyanoferrate Based Glucose Biosensor

• Main Authors: Wu, Yi-Cong, 吳易聰
• Other Authors: Lin,Meng-Shen
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/51752487249324959970

碩士 === 淡江大學 === 化學學系 === 84 === Mixed-valence Cluster of Cobalt(II) hexacyanoferrate has an electron transfer chain and it is suitable for the development of a glucose biosensor.The glucose oxidase containing about Cobalt(II) hexacyanoferrate the biosensor was prepared by immersing rotating glassy carbon electrode into solchaon containing 15mM Co(NO3)2.6H2O and 15mM K3Fe(CN)6 and glucoseoxidase at -0.05V(vs.Ag/AgCl) for 1 hr. The electrode was depositedon the same condition for 4 hrs in 15mM Co(NO3)2.6 H2O ane 15mM K3Fe(CN)6 without glucose oxidase. The enzyme( glucose oxidase) was codeposited together with Cobalt(II) hexacyanoferrate onto the rotating glassy carbonelectrode upon the The addition of glucose, the glucose oxidase generates hydrogen peroxide. The H2O2 oxidize the reduced Cobalt(II) hexacyanoferrate when the electrode electrode potential was applied at +0.0V. Infraredspectra for Cobalt(II) hexacyanoferrate exhibited absorption peak at2080,593 and 457 cm- for -CN-,-FeC and -FeCN- stretching frequency, corr-espond to previously of literature. Thus, the reduced current is proportional to the concentration glucose. The linearity of the response is up to 5mM(with correlation coefficient 0.9999). The sensitivity fo the electrode is 0.733 microamp./mM-cm2. The extimated detection limit is 2×10-6M. The response time is 14.5 sec. The twenty repetitive detection 0.5mM glucose indicated stantard deviation 4.0246%, There is no interferenceobserved, such as ascorbic acid, uric acid, galactose, cystein , tyrosine, dopamine,acetaminophen and 1,4-dihydroxyquinone. The Cobalt(II)hexacyanoferrate film on the electrode surface can avoid fauling from protein.