| Summary: | 碩士 === 台北醫學院 === 醫學研究所 === 84 === AbstractIn order to completely utilize the polysaccharide
isolated from the fruiting body of ''Ling Zhi'', this study used
the most common cultured species in Taiwan, the mushroom
Songshan lingzhi (Ganoderma tsugae,Murrill), as its material. We
extracted three polysaccharides by different processes such as
ultrafiltration, semimembrane dialysis, and lyophilization. The
purpose of the study is to compare the chemical and antitumor
properties of the polysaccharide produced by 1. F-I (water
soluble polysaccharide), 2. F-II (alkali soluble
polysaccharide), and 3. F-III (the deacetylated cell wall
skeleton). The chemical analysis included total sugar assay,
sugar component, ratio of the sugar component, and molecular
weight. The methods involved in the analysis are : TLC, HPLC,
Gel-filtratiion, and Acid hydrolysis. Antitumor activities of
these polysaccharides were assayed by challenging Sarcoma 180
tumor cells to Balb/c mice and prior to which the
polysaccharides were administrated into cadal vein,
intravenously. The pulmonary nodules of S-180 induced by
metastasis were counted by flow cytometer and naked eyes. In
addition, the lymphocyte subsets in peripheral blood were
measured in respect to the ratio of T, T4, T8, B, and
Macrophage. In this experiment, we tried to utilize the residue
of Ling Zhi which is used to be dumped. In the whole process,
two additional polysaccharides were produced in large quantity
and we expect these polysaccharides functioned as well as that
from water soluble one. These results indicate that F-I and F-II
had no much difference in their components. They were the
polymers of glucose and N-acetyl-D-glucosamine (or glucosamine).
The major component of F-III is glucosamine (60%). In addition,
to the G2+M(%) phase of murine S-180 sarcoma cells, the
inhibitory effect is F-III (38.28%) > F-I (64.30%) > F-II
(79.84%) > control (100%).
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