Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium
碩士 === 中國醫藥學院 === 醫學類 === 85 === Leukemia inhibitory factor(LIF) is a multi-functional cytokine that plays pleiotropic activities in varioustissues. It has been demonstrated to be essential for blastocyst implantation in mice. LIF transcrip...
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ndltd-TW-085CMCH05360012015-10-13T12:14:44Z http://ndltd.ncl.edu.tw/handle/45327145073962224465 Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium 人類白血癌抑制因子基因在子宮內膜細胞表現之研究 Huang, Yi-Ping 黃怡萍 碩士 中國醫藥學院 醫學類 85 Leukemia inhibitory factor(LIF) is a multi-functional cytokine that plays pleiotropic activities in varioustissues. It has been demonstrated to be essential for blastocyst implantation in mice. LIF transcript is restricted in endometrial glands of uterus in adult mice. The level of LIF mRNA fluctuated the estrous cycle and reached to peak after ovulation(specially on day 4 of pregnancy). Expression of human LIF gene in uterus has been demonstrated by Northern blot and RT-PCR analysis recently. Glandular epithelial cells of the mid-luteal phase, at the expected time of implantation in the human, produced significantly more than that of the other stages. The role of LIF on human reproduction needs to befurther defined. Therefore, we have analyzed the expression of LIF gene in human uterine endometrium.Specimens were obtained by biopsy from volunteer participants. The anti-sense RNA probes labeled with35S was employed for in situ hybridization. The anti-sense RNA probes labeled with 32P wasemployed for RNase protection. Our data showed that LIF gene was expressed with a low level inglandular and luminal epithelium, and stroma cells at proliferative phase. In early secretary phase, theexpression of LIF gene in laminal and glandular epithelium was significantly elevated . Unlike the previous results obtained in mice, our preliminary results demonstrated that LIF gene expression is not restricted in glandular epithelium of human uterus. The results suggested that maternalexpression of LIF restricted in luminal and glandular epithelium of uterus might contribute to autocrineand/or paracrine regulation of uterine proliferation. It could be essential for providing an ideal environment accessible for embryo implantation. Hsu Li-Wei 徐立偉 1997 學位論文 ; thesis 76 zh-TW |
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碩士 === 中國醫藥學院 === 醫學類 === 85 === Leukemia inhibitory factor(LIF) is a multi-functional cytokine
that plays pleiotropic activities in varioustissues. It has been
demonstrated to be essential for blastocyst implantation in
mice. LIF transcript is restricted in endometrial glands of
uterus in adult mice. The level of LIF mRNA fluctuated the
estrous cycle and reached to peak after ovulation(specially on
day 4 of pregnancy). Expression of human LIF gene in uterus has
been demonstrated by Northern blot and RT-PCR analysis recently.
Glandular epithelial cells of the mid-luteal phase, at the
expected time of implantation in the human, produced
significantly more than that of the other stages. The role of
LIF on human reproduction needs to befurther defined. Therefore,
we have analyzed the expression of LIF gene in human uterine
endometrium.Specimens were obtained by biopsy from volunteer
participants. The anti-sense RNA probes labeled with35S was
employed for in situ hybridization. The anti-sense RNA probes
labeled with 32P wasemployed for RNase protection. Our data
showed that LIF gene was expressed with a low level inglandular
and luminal epithelium, and stroma cells at proliferative phase.
In early secretary phase, theexpression of LIF gene in laminal
and glandular epithelium was significantly elevated . Unlike the
previous results obtained in mice, our preliminary results
demonstrated that LIF gene expression is not restricted in
glandular epithelium of human uterus. The results suggested that
maternalexpression of LIF restricted in luminal and glandular
epithelium of uterus might contribute to autocrineand/or
paracrine regulation of uterine proliferation. It could be
essential for providing an ideal environment accessible for
embryo implantation.
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author2 |
Hsu Li-Wei |
author_facet |
Hsu Li-Wei Huang, Yi-Ping 黃怡萍 |
author |
Huang, Yi-Ping 黃怡萍 |
spellingShingle |
Huang, Yi-Ping 黃怡萍 Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium |
author_sort |
Huang, Yi-Ping |
title |
Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium |
title_short |
Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium |
title_full |
Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium |
title_fullStr |
Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium |
title_full_unstemmed |
Investigation of expression of human leukemia inhibitory factor( LIF) gene in uterine endometrium |
title_sort |
investigation of expression of human leukemia inhibitory factor( lif) gene in uterine endometrium |
publishDate |
1997 |
url |
http://ndltd.ncl.edu.tw/handle/45327145073962224465 |
work_keys_str_mv |
AT huangyiping investigationofexpressionofhumanleukemiainhibitoryfactorlifgeneinuterineendometrium AT huángyípíng investigationofexpressionofhumanleukemiainhibitoryfactorlifgeneinuterineendometrium AT huangyiping rénlèibáixuèáiyìzhìyīnzijīyīnzàizigōngnèimóxìbāobiǎoxiànzhīyánjiū AT huángyípíng rénlèibáixuèáiyìzhìyīnzijīyīnzàizigōngnèimóxìbāobiǎoxiànzhīyánjiū |
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