| Summary: | 碩士 === 國立中興大學 === 園藝學系 === 85 === Morphological traits and randomly amplified polymorphic DNA
(RAPD) markerswere used to study the identification and
discrimination of Cymbidium species.The purposes of this study
were to establish the molecular markers for discrimination of
the Cymbidium spp. and cultivars and to explore the
relationships of genetic linkage among the Cymbidium spp. and
cultivars. The data of phenotype characters and growth habit
showed that the tenCymbidium spp. can be discriminated followed
the order of serrulated marginof leaf, leaf shape, leaf size,
and growth habit. However, fifteen cultivars ofCymbidium can
only be parly discriminated with leaf stripes and leaf shape.
Twenty DNA primers, which generated clear and discriminative
polymorphicDNA fragments, were selected from 300 random primers
to discriminate ten Cymbidium species. Of 79 polymorphic DNA
fragments, 46 fragment were species-specific and 33 fragments
were presented between two Cymbidium spp. Therewere averaged
12.5 amplified DNA fragments a-nd 3.9 DNA polymorphic
markersgenerated by each primer. The results of linkagof cluster
analysis revealedthat the ten Cymbidium spp. can be classified
into four groups, the first group includes terrestrial plants of
C. rubrigemmum, C. ensifolium var. misericors, C. karan, C.
sinense and C.tortisepalum, the second group includes C.
lancifolium form aspidistrifolium and C. formosanum, the third
group includes epiphytic plants of C. Sensation and C. pumilumm,
and the fourth group includes C. dayanum var. austro-japonicum
with the lowest similar-ity with other Cymbidium spp. Eleven DNA
primers were selected from 300 random primers to discriminate
fifteen cultivars of Cymbidium. Of 21 polymorphic DNA fragments
which discriminated eleven cultivars of Cymbidiu sinense, 11
fragments were cultivar-specific and 10 were shared among two to
four cultivars. There were averaged 11 amplified DNA fragments
and 1.9 DNA polymorphic markers gener-ated by each primer. The
results of linkage cluster analysis revealed that the eleven
cultivars of Cymbidium sinense can be classified into four
groups, the first group includes C. sinense and `taur-ji', the
second group includes `rueih-baau', `yarng-ming-jiin', `jin-
shan', `shyueh-bair-jaau' and`shyuh-huarng', thethird group
includes ` shyr-mern', and the fourth groupincludes `jin-huah-
shan' and `shih-ba-hsiieh-shih'.
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