| Summary: | 碩士 === 國立交通大學 === 應用化學研究所 === 85 === A mathematical modeling is established in this study to correlate supercritical fluid chromatography (SFC) in aqueous stationary phase with supercritical fluid extraction (SFE) in aqueous matrices. A solar coaxial countercurrent chromatography was applied for both SFC and SFE experiments. The extraction vessel, i.e. the column for SFC, was mathematically divided into limited layers. During the extraction, each layer was considered undergoing a chromatographic process. The plate heights of all the layers was assumed equal throughout the column. The capacity factor and peak broadening for each "imaginary" chromatographic peak were calculated by the true SFC experimental data. Accordingly, the analyte recovery curve of SFE can be simulated with SFC data using this model. Since operations of SFC are more straightforward than those of SFE, optimization of SFE may be more easily achieved with this mathematical correlation.
The simulated data of analytes with capacity factor greater than 0.9 mathch the experimental results very well. Deviations become gradually greater while capacity factors of analytes decrease. A rationale was proposed to successfully interpret this deviation trend.
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