| Summary: | 碩士 === 國立臺灣大學 === 農業化學系 === 85 === Several strains of marine microalgae, including Pavlova lutheri
(CCMP 1325), Pavlova salina, Isochrysis galbana TK1,
Isochrysis galbana TK2, Isochrysis sp. (CCMP 1324), Isochrysis
sp. (CCMP 463), Nannochloropsis oculata, Chlorella minutussima
(UXTE 2341) were cultured and analyzed for their fatty acid
composition. Isochrysis galbana TK2 and Pavlova lutheri (CCMP
1325) were species found to be rich in EPA and DHA contents and
subjected to cultured test. Cultural conitions were examined for
the optimal conditions for production, on salt concentrations,
temperature, the concentration of sodium aceteat for carbon
source, and the concentration of sodium glutamate and urea for
nitrogen sources. Eventually, we eluadate the effect of
environment factors on algal biomass DHA productions.The
experimental results showed that an optimal culturing of
Isochrysis galbana TK2 were 3.2﹪salnity , temperature 25℃.
Sodium glutamate was the best nitrogen source. The suitable
concentration was 10 mM, and the dry weight can reached 0.59 g
per litre culture, the DHA production can reach 12.04 mg per
litre culture. The suitable concentration of sodium acetate for
mixtrophic culture of Isochrysis galbana TK2 was 10 mM. The dry
weight can reach 0.54 g per litre culture, and DHA can reach
10.85 mg per litre culture. The optimal culturing of Pavlova
lutheri (CCMP 1325) were 3.2﹪salnity, temperature 25℃. The
best nitrogen source was sodium nitrate, and autotrophic
cultulture was better than mixotrophic culture. When the
concentration of sodium glutamate and urea increasing, the
contects of DHA in cells were decreasing in Isochrysis galbana
TK2 and Pavlova lutheri (CCMP 1325) culture. But when we added
sodium acetate into Isochrysis galbana TK2 and Pavlova lutheri
(CCMP 1325) culture, the contects of DHA in cells were
increasing. f marine microalgae, including Pavlova lutheri (CCMP
1325),
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