| Summary: | 碩士 === 國立臺灣大學 === 醫事技術學系 === 85 === The aim of this study was to understand the prevalence of
anti-GAD65 (anti-glutamic acid decarboxylase）, one of the
major autoantibodies that exist in insulin-dependent diabetes
mellitus（IDDM）, and to analyse the relationship between HLA
class II molecules and anti-GAD65. In this study we established
96-well format immunoprecipitation radioligand method to detect
anti-GAD65 antibody, and established SSP-PCR（sequence-specific
primer-PCR） method for typing HLA class II gene.
The study population consisted of 167 IDDM patients, 125
relatives of IDDM（parents and siblings）， and 105 healthy
control. The results showed that：（1）The prevalence
of anti-GAD65 in Taiwanese IDDM was 58.33﹪. The prevalence of
anti-GAD65 decreased with the increase of the duration of
disease, and at the same time, the titer of anti-GAD65 declined
with the increase of the duration of disease.(2) Two of the HLA
haplotypes：DRB1*17-DQA1*0501-DQB1*0201,DRB1*04-DQA1*0302-
DQB1*0401 showed strong association with IDDM(p<0.05). However,
three of the HLA haplotype :DRB1*11-DQA1*0501-DQB1*0301,DRB1*12-
DQA1*0601-DQB1*0301,DRB1*14-DQA1*0101- DQB1*0503 were protective
against IDDM（p＜0.05）.（3）HLA genotypes, DRB1*09/09 and
DQB1*0303/0303 were associated with an increase in the frequency
of anti-GAD65（p＜0.05）. Nevertheless, DRB1*04-DQA1*0302-
DQB1*0401 could be correlated wition will be necessary.（5）The
HLA haplotype： DRB1*17-DQA1*0501-DQB1*0201 had association with
early age of onset.（6）The DQα1（ Arg52）molecules had larger
effect than DQβ1(Asp57）molecules to susceptibility of IDDM.
In this study, we established 96-well format immunoprecipitation
radioligand method to detect anti-GAD65 antibody. It's the first
time to analyse the prevalence of anti-GAD65 in Taiwanese IDDM,
and to analyse the relationship between the prevalence and
titer of anti-GAD65 with HLA class II molecules.
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