| Summary: | 碩士 === 國立臺灣大學 === 獸醫學系 === 85 === M. fortuitum complex (MFC), 包括M. fortuitum、M. peregrinum、M.
fortuitum third biovariant
sorbitol positive、M. fortuitum third biovariant sorbitol
negative、M. chelonae、M. abscessus
及M. chelonae-like organism等7種具病原性的細菌。可引起人之角膜炎
、關節炎與軟組織化膿、及慢性肺炎等疾病。
罹患後天免疫不全症候群之病人亦常受MFC感染。1992年本研究室對某一
乳牛場環境調查發現, 其牛隻結核菌素非特異性反應率偏
高, 而其環境中普遍存在MFC, 遂懷疑MFC為造成其結核菌素非特異性反應
的原因。鑑於臺灣地區對MFC在自然界及動物界之分佈
尚無文獻可稽, 本論文以乳牛場為對象來研究臺灣地區MFC的生態分佈。
從1995年10月迄1996年7月由全島乳牛場採集場內
飲用水及土壤各114件、乳牛糞便228件, 共456件檢體, MFC檢出率
為46%, 其中以土壤檢出率最高, 佔79.8%。
鑑於乳牛場環境中MFC的高檢出率, 再由野外未耕地採樣分離MFC, 其分離
率僅有25%。由此推論可能是乳牛場環境適合MFC之生長。
分離到之210株MFC, 以生化特性及PCR-RFLP見定其菌種, 其中以M.
fortuitum居多, 佔76%。故選定M. fortuitum為研究對象, 由
北、中、南、東區及未耕地四區分離之M. fortuitum各選取20株進行藥物
感受性試驗, 質體相分析及核糖體DNA限制脢片段
多型性分析, 來比較不同地區之M. fortuitum在質體相及核糖體DNA限制
脢圖譜方面, 差異不大。而藥物感受性方面, 乳牛場的分離
株較野外未耕地分離株具抗藥性。
Among the rapidly growing mycobacterium, M. fortuitum、M.
peregrinum、M. fortuitum third biovariant
sorbitol positive、M. fortuitum third biovariant sobitol
negative、M. chelonae、M. abscessus and
M. chelonae-like organism are recognized as the potential
pathogen which may cause keratitis,
anthritis, superficial dermatitis, chronic pulmonary disease,
or abscess of soft tissue in human.
Because they share a number of characteristics and are
associated with similar types of infections,
they were referred to collectively as the M. fortuitum complex
(MFC). In order to understand the
ecological distribution of MFC in Taiwan, we collected soil,
drinking water and cow stool from
dairy farms around the island for isolation and isentification
of the organism. The total isolation
rate of mycobacteruia was 46%. The isolation rates from
drinking water, soil and coe stool were 27.2%,
79.8% and 38.6% respectively. The isolation rate of soil from
uncultivated land was only 25%.
Mycobacterium fortuitum was the major isolate among our study
(76%). One hundred such isolates
from dairy farm and uncultivated land were tested for their
antimicrobial susceptibility against
10 common drugs in vitro. These isolates were also studied for
plasmid profiles and ribosomal ribonucleic
acid (rRNA) gene restriction patterns. The drug resistance
patterns show that the isolates from dairy farms
were more resist than those of uncultivated lands. The plasmid
profiles and rRNA gene restriction patterns of
teh isolates from dairy farms were not significant different
from those from uncultivated lands.
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