| Summary: | 碩士 === 中國醫藥學院 === 中國醫藥研究所 === 86 === The present study was investigated the anxiolytic effects of Kan-Mai-Ta-
Taso-Tang by the animal anxiety models: two-way active avoidance,
black/white chamber, and elevated plus-maze. We also investigated the
action mechanism of KM via combining with the drug altered the 5-HT
neuron activities or using biochemical method. KM ( 0.3-2.0 g/kg, p.o. )
decreased the rats'' response counts of unconditioned stimulus and
intertrial crossing in two-way active avoidance. In the elevated plus-maze,
KM ( 0.3-1.0 g/kg, p.o. ) increased the percentage of the time spent in the
open arms and entries, and decreased the percentage of the time spent in
the closed arms and entries. However, KM ( 2.0 g/kg, p.o. ) prolonged the
percentage of the time spent of mice in the closed arms. For mice in the
black/white chamber, KM prolonged the time spent in the white chamber
and decreased the time spent in the black chamber. KM ( 1.0, 2.0 g/kg,
p.o. ) decreased the locomotor activities of mice.
KM ( 0.3 g/kg, p.o. ) blocked the anxiogenic effect induced by 5-HTP,
and enhanced the anxiolytic effects induced by PCPA, buspirone, p-MPPI
and ritanserin in mice. The concentrations of monoamines and their
metabolites in the brain stem of mice were detected by using High-
performance liquid chromatography. KM decreased the 5-HT, NE, DA
and 5-HIAA levels, and increased the VMA and HVA levels.
From the above results, the mechamisms on the anxiolytic effects of KM
may be related to the decrease in the 5-HT turnover rate via activating the
activity of the presynaptic 5-HT1A autoreceptors and decreasing the
activities of the postsynaptic 5-HT1A , 5-HT2 receptors
|
|---|
