Investigation of the Expression of Protein Kinase C Isoforms in Tumour Tissues

• Main Authors: Chang, Ai-Chi, 張愛琪
• Other Authors: Liu, Jer-Yuh
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/43756105825823573933

碩士 === 中山醫學院 === 生物化學研究所 === 86 === 　　(1)It is well known that decidualization is correlated with the implantation. However, the mechanism of the decidualization remain understand. So, the investigating of the development of deciduomata is worth to do. 　　In this study, we investigated the correlation between decidualization and protein kinase C ( PKC ) expression. We determined the expression of PKC isoforms during trauma-induced decidualization. The findings revealed that at least fie PKC isoforms（α , δ , ζ , ι and λ） were present in both control and decidualized tissue.After trauma-stimulation, PKC α was down-modulated in the deciduomata but not in the myometrium. Down-modulation was compatible with the increase in cell mitosis which reached a maximum at 2-3 days. On the other hand, PKC ζ was not down-modulated, It was increased both inthe deciduomata and myometrium, and paralleled the freqtuence of decidual cell mitosis. The PKC isoforms of ζ,ι and λ were also increased, but they were associated with the depression of cell mitosis. Immunohistochemistry assay confirmed the various expression of PKC isoforms.Therefore, these findings suggested that the variable expression of PKC isoforms in trauma-induced decidualizing tissue in pseudopregnant rats may be involved in the modulation of decidual cell growth. 　　(2)In our previous study, we had found that the reduced membrane-PKC activity was associated with the malignancy of liver cancer. This study was investigated the expression of PKC isoforms in human liver cancer tissue. The samples abtained from Department of surgery of Changhua Christian Hospital, were subfracted into cytosolic fraction and membrane fraction, and analyzed by western blot. The result showed that the membrane-PKC□ in cancer tissue was significantly decreased as comparing with that in the adjancent normal tissue, while the cytosolic PKC□was not different. This data was paralleled with PKC activity in human liver cancer. Whereas, both PKC□ and PKC□ were significantly increased in both cytosolic and membrane fractions. Moverover, these various expression of PKC □ PKC □ and PKC□were observed in all of 8 patients.Thus, we suggested that the various expression of PKC isoforms amy be correlated with the development of the human liver cancer.