Heterologous Expression of Penicillin Acylase in E. coli by PCR- Cloning pac Gene from Providencia rettgeri ATCC31052
碩士 === 逢甲大學 === 化學工程研究所 === 86 === PAC酵素不僅具工業應用價值,在於學術上也是一個很好的研究對象。本 研究利用PCR技術將Providencia rettgeri ATCC31052的pac基因植株,並 將其副植株於tac啟動子的表現載體上,建構出數個重組質體以於E. coli 生物系統中進行基因之異形表現(heterologous expression)。對於PAC酵 素生成的環境調節方面,例如誘導劑與不同種...
Main Authors: | Lin, Ming-I, 林明毅 |
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Other Authors: | C.Perry Chou |
Format: | Others |
Language: | zh-TW |
Published: |
1998
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Online Access: | http://ndltd.ncl.edu.tw/handle/46330078520033018207 |
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