STUDIES ON THE CYTOTOXIC CONSTITUENTS OF FORMOSAN MALLOTUS REPANDUS

• Main Authors: WANG, LI-WEN, 王麗雯
• Other Authors: CHUN-NAN LIN
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/79059634077564327976

碩士 === 高雄醫學院 === 天然藥物研究所 === 86 === Bioactivity-guided fraction of extracts the root barks of Chinese herb, Mall otus repandus, led to the isolation of three new triterpenoids of the ursan ty pe,3α-hydroxy-13β- ursan-28,12α-olide 3-benzoate(1), ursan-3α-ol(3) and 3 α- hydroxy-28β-methoxy-13β-ursan-12α,28-epoxy 3-benzoate(4); as well as four known compounds,3-oxo-13β-ursan-28,12α-olide(2),3 α-hydroxy-13β-ursan-28, 12α -olide(5), ursolic acid(8) and bergenin(9), whose structure identified by chemical and spectral (IR,UV,MS,NMR)methods. In addition, the characterization of the unknown compounds 6 and 7 are still on going. Ursane type triterpenoid derivatives 1, 8 and 9 were screen for cytotoxic activities against KB,PLC/PRF/5, T24,HT3,SiHa and Caski tumor cells in vitro.The results indicated that ursolic acid(8) showed significant cytotoxicity against KB, PLC/PRF/5 cells, 1 showed marginal effect against several human tumor cell lines. While compound 9 were inactive against KB,T24,HT3,SiHa,PLC/ PRF/5 and Caski cell lines. The isolation of other bioactive constituents are still undertaken.