| Summary: | 碩士 === 國立交通大學 === 生物科技研究所 === 86 === Sulfation in biological system plays important roles in
regulating structure and function of macromolecules,
availability of hormones and neurotransmitters, activation and
deactivation of xenobiotics, and elimination of end products of
catabolism. Sulfotransferase catalyze all the known biological
sulfation which involves the transfer of a sulfuryl group from a
common sulfate donor, a nucleotide called 3'-phospho adenosine
5'-phosphosulfate (PAPS). Another nucleotide, 3'-phospho
adenosine 5'-phosphate (PAP)A double mutant (K65E+R68G) exhibits
over 500-fold increase in Km for PAP with Vmax remained the same
（6）. Adenosine 5'-monophosphate (AMP), Adenosine 2',
3'-cycliphosphate 5'-phosphate and Adenosine 2', 5'-bisphosphate
were shown to replace PAP as cofactor for sulfuryl group
transfer（24）. However neither mutant's Km nor Vmax of these
nucleotides differ significantly with that of wild type's.
According to the study of Yoshimitsu, et al, Lys 65 and Arg68
were not involved in enzyme PAP binding site. But w
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