The Crystal Structure Determination of Metal(II) Complexes and The Crystal Growth of Protein

• Main Authors: Shui, Wei-Ze, 徐瑋擇
• Other Authors: Lu Tian-Huey, Lin Guey-Lin
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/94036086937973182836

碩士 === 國立交通大學 === 物理研究所 === 86 === This paper includes two parts. One is to determine the structure of the single crystal of four Metal(Ⅱ) Complexes by X-ray diffraction. The other is the method and process in the crystal growth for the protein of CardiotoxinⅣ First: The four crystals were provided by Prof. Chung-Sun Chung in the Chemistry Department of NTHU. We determined the structure of the 4 single crystals by X-ray diffraction. Of the four complexes, copper(Ⅱ) ion is five coordinated with geometry of distorted trigonal bipyramid structure in the first crystal. Copper(Ⅱ) ion has six coordinations with distorted square bipyramid in the second crystal. In the third, nickel(Ⅱ) ion has six coordinations with four N atoms equatorial and N atoms of the isothiocyanate groups axial, the same geometry as the second crystal. Nickel(Ⅱ) ion is four-coordinate with four nitrogen atoms in the macrocyclic plane in the last crystal.Second: The purified material of CardiotoxinⅣwas provided by Prof. C.C. Yang in the Department of Life Science of NTHU. We used hanging drop of vapor diffusion method to grow protein of crystal. We tried to screen the best condition for the crystallization of CadiotoxinⅣ from 48 conditions.