| Summary: | 碩士 === 國立臺灣大學 === 農業化學系研究所 === 86 === The MRL/lpr mice develop a syndrome that resembles human systemic
lupus erythematosus ( SLE ), including production of autoantibodies and
formation of immune complexes. They have a strikingly short life span and die
at the age of 6~8 months secondary to renal failure. The purpose of this study
was to investigate the effects of high levels of vitamin E supplementation on
the autoimmune disease courses. Ninety weanling MRL/lpr female mice were
randomly divided into three groups and respectively fed on an AIN-76 diet
containing 5% fresh soybean oil ( Control ), control diet supplemented with
vitamin E at 10 times levels of AIN-76 ( E10 ), or 20 times
levels ( E20 ).After
14 weeks, half of the mice were killed for in vitro experiments at 5 months of
age.
The results showed that the relative weights of spleen and kidney were
reduced in E10 and E20 groups. Supplementation with all-rac--tocopheryl
acetate elevated the -tocopherol content and reduced TBARS in liver and
kidney. With respect to the antioxidant enzyme activities, the hepatic SOD and
GSH-Px activity were significantly ( p<0.05 ) higher in E20 group and the
renal SOD activity was significantly higher in E10 group. The E10 and E20
groups had lower IL-6 and PGE2 secreted from peritoneal cells than the control
group. PGE2 production by spleen cells was also reduced in E10 and E20
groups. But there were no effects on TNF-、IL-6 and IL-10 production.
In conclusion, vitamin E supplementation decreases the oxidative stress
and the PGE2 production from splenocytes and peritoneal macrophage, but it
neither reduce the autoantibody production nor prolong the life span through
the Th1、Th2 cells regulation in MRL/lpr mice.
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