| Summary: | 碩士 === 國立臺灣大學 === 免疫學研究所 === 86 === Increasing prevalence of pertussis infection has been reported in
recent years. Both pertussis toxin (PT) and filamentous hemagglutinin
(FHA) have been used as the components of acellular vaccine for
pertussis infection. Several studies suggested that PT can induce
a very strong proliferative response for human lymphocytes and to
lesser extent, murine lymphocytes. To further characterize the
cellular immune response, intracellular staining of DNA with DNA
dye and anti-BrdU antibody, antigen-reactive T cell frequency by
limiting dilution and a-MHC-II mAb blocking effect of peripheral
blood mononuclear cells (PBMCs) were analyzed. The data suggested
both the proliferative response and antigen-reactive cell frequency
of PT stimulated PBMCs were similar to that of Con A stimulation,
which was much higher compared to that of FHA stimulation. In
addition, PT can also induce a good cellular proliferative response
of cord blood mononuclear cells which are regarded as a population
of naive cells. With further characterization of PT-specific
cellular immune response might shed light into further design of
pestussis vaccine and application of PT as an immunological adjuvant.
|
|---|
