Characterization of Cellular Immune Response to Pertussis Toxin

• Main Authors: Huang, Chia-Lin, 黃嘉琳
• Other Authors: Lin,Rong-Hwa
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/83354072131490844040

碩士 === 國立臺灣大學 === 免疫學研究所 === 86 === Increasing prevalence of pertussis infection has been reported in recent years. Both pertussis toxin (PT) and filamentous hemagglutinin (FHA) have been used as the components of acellular vaccine for pertussis infection. Several studies suggested that PT can induce a very strong proliferative response for human lymphocytes and to lesser extent, murine lymphocytes. To further characterize the cellular immune response, intracellular staining of DNA with DNA dye and anti-BrdU antibody, antigen-reactive T cell frequency by limiting dilution and a-MHC-II mAb blocking effect of peripheral blood mononuclear cells (PBMCs) were analyzed. The data suggested both the proliferative response and antigen-reactive cell frequency of PT stimulated PBMCs were similar to that of Con A stimulation, which was much higher compared to that of FHA stimulation. In addition, PT can also induce a good cellular proliferative response of cord blood mononuclear cells which are regarded as a population of naive cells. With further characterization of PT-specific cellular immune response might shed light into further design of pestussis vaccine and application of PT as an immunological adjuvant.