| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程技術研究所 === 86 ===
This lack of insulin inside the body of diabetic patient results in abnormal liver metabolism, as a consequence a lot of 1,2-propanediol will accumulate in the blood. This work discusses the application of glycerol dehydrogense for trace 1,2 - propanediol adsorption. In the aqueous solution, 1,2- propanediol can be adsorbed by glycerol dehydrogense via the analysis fo gas chromatograph. The adsorbed 1,2- propanediol is oxidized to lactaldehyde, after adding it's cofactor NAD+ into the solution. To apply the piezoelectric sensor for the detection of 1,2- propanediol in the vapor phase, glycerol dehydrogenase was immobilized to a piezoelectric quartz cryctal. As determined by the frequency decrease of the piezoelectirc quartz crystal, glycerol dehydrogenase shows the best capability to adsorb 1,2 - propanediol in the condition of 5% relative humidity. In comparsion with alcohol dehydrogenase, serum-albumin bovin and glycine, glycerol dehydrogenase also shows the highest adsorption rate for 1,2 - propanediol.
The glycerol dehydrogenase immobilize piezoelectric quartz cryatal was regenerated by water flushing or nitrogen purging. After repeated use for six times, the adsorption capability and adsorption rage of the glycerol dehydrogenase immobilized crystal decrease to 60% and 78%, repectively. In the condition of adsorption of 1,2- propanediol under 100% relative humidity, the immobilized glycerol dehydrogenase crystal did not show any frequency decrease. However, a unique frequency variation was noted for the immobilized glycerol dehydrogenase crystal when nitrogen purging was employed.
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