Analysis of Rhubarbs by LC/ES/MS
碩士 === 東吳大學 === 化學學系 === 86 === Rhubarbs have been used in Chinese herbal medicine for a long time. Five active components, sennoside A and B, rhein, emodin, and aloe-emodin, were already being separated from this herbal drug. However, having the tendency to become unstable upon the application of...
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ndltd-TW-086SCU000650062015-10-13T17:34:40Z http://ndltd.ncl.edu.tw/handle/85587560184630328229 Analysis of Rhubarbs by LC/ES/MS 利用液相層析/電灑/質譜儀檢測大黃樣品中之主要成份 Lin Hung-Jian 林宏建 碩士 東吳大學 化學學系 86 Rhubarbs have been used in Chinese herbal medicine for a long time. Five active components, sennoside A and B, rhein, emodin, and aloe-emodin, were already being separated from this herbal drug. However, having the tendency to become unstable upon the application of heat, they are difficult to determine by GC and other techniques. Although the active components of rhubarbs can be separated by HPLC and detected by photodiode array detector, their complexity and low content make identification by photodiode array detector or conventional UV difficult.In this thesis, the use of LC/ES/MS to analyze these components was investigated. An analytic method is developed to separate and identify the components of rhubarbs by using C18 column and gradient elution to first separate the components that are then determined by ES/MS. In addition, the effect CapEx, mobile phase composition, mobile phase additives, and flow rate have on ES/MS detection as well as the linearity of LC/ES/MS and detection limit are evaluated. The application of this newly developed LC/ES/MS method for the examination of a variety of rhubarb extracts was demonstrated. Fun Ming-Ren 傅明仁 1998 學位論文 ; thesis 0 zh-TW |
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碩士 === 東吳大學 === 化學學系 === 86 === Rhubarbs have been used in Chinese herbal medicine for a long time. Five active components, sennoside A and B, rhein, emodin, and aloe-emodin, were already being separated from this herbal drug. However, having the tendency to become unstable upon the application of heat, they are difficult to determine by GC and other techniques. Although the active components of rhubarbs can be separated by HPLC and detected by photodiode array detector, their complexity and low content make identification by photodiode array detector or conventional UV difficult.In this thesis, the use of LC/ES/MS to analyze these components was investigated. An analytic method is developed to separate and identify the components of rhubarbs by using C18 column and gradient elution to first separate the components that are then determined by ES/MS. In addition, the effect CapEx, mobile phase composition, mobile phase additives, and flow rate have on ES/MS detection as well as the linearity of LC/ES/MS and detection limit are evaluated. The application of this newly developed LC/ES/MS method for the examination of a variety of rhubarb extracts was demonstrated.
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author2 |
Fun Ming-Ren |
author_facet |
Fun Ming-Ren Lin Hung-Jian 林宏建 |
author |
Lin Hung-Jian 林宏建 |
spellingShingle |
Lin Hung-Jian 林宏建 Analysis of Rhubarbs by LC/ES/MS |
author_sort |
Lin Hung-Jian |
title |
Analysis of Rhubarbs by LC/ES/MS |
title_short |
Analysis of Rhubarbs by LC/ES/MS |
title_full |
Analysis of Rhubarbs by LC/ES/MS |
title_fullStr |
Analysis of Rhubarbs by LC/ES/MS |
title_full_unstemmed |
Analysis of Rhubarbs by LC/ES/MS |
title_sort |
analysis of rhubarbs by lc/es/ms |
publishDate |
1998 |
url |
http://ndltd.ncl.edu.tw/handle/85587560184630328229 |
work_keys_str_mv |
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