Chromatographic Analysis of Phenylpropanolamine Using Synthesized Polymer Sorbents

• Main Authors: Jinn Yuan Kuo, 郭進原
• Other Authors: Wen Chien Lee
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/52403256943279411525

碩士 === 國立中正大學 === 化學工程研究所 === 87 === Abstract More than a half of pharmaceutical drugs on the whole market are asymmetric molecules. About 90% of those are administered as racemates. It is not a surprise that the two enantiomers of a racemic drug might interact with the biological system differently. One of the enantiomers may have a pharmacologically different or unwanted side effect. If we separate one from another enantiomer efficiently, we can take less side effects and a less amount of drugs. We used here the latest molecular imprinting technology to prepare a specific affinity sorbent that could be use for the separation of a sympathomimetic chiral drug. In this study, we prepared the molecular imprinting polymers using the method of bulk polymerization. The resultant polymers were crushed and sieved to be the stationary phase, which could separate the mixture consisting of phenylpropanolamine enantiomers and their impurity, aminopropiophenone. In order to search for the best conditions, the polymers were prepared by employing different mole fractions of the crosslinking monomer based on total amount of monomers, reaction temperatures and volume ratios of solvent to all monomers. The mobile phase composition for the chromatography was also studied. From the experimental results, we found that the best conditions for polymer preparation are that the reaction temperature is 4 ℃, the fraction of crosslinking monomer is 80 mol%, and the fraction of solvent is 2.213. The best mobile phase composition is keeping water:acetic acid:acetonitrile at 2.5:5:92.5. We were able to use a 25 cm-long column to separate effectively the enantiomers of phenylpropanolamine and aminopropiophenone. When the 5 μl sample containing 1 mg/ml (±)phenylpropanolamine was applied to the column, a separation with the separation factor of 3.27 and resolution of 3.8 was achieved. The chromatographic efficiency may be improved by using other approachs to prepare spherical molecular imprinting particle with a uniform distribution in size. A large-scale chromatography system based on the results obtained in this work could also be established.