Molecular Mechanisms of Responder and Non-responder to Electroacupunctural Analgesia-C-fos Expression in the Rat Spinal Cord Dorsal Horn

• Main Authors: Chia-Ni Lee, 李佳霓
• Other Authors: Jaung-Geng Lin
• Format: Others
• Language: en_US
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/47951282580730232158

碩士 === 中國醫藥學院 === 中國醫學研究所 === 87 === The purpose of this study is to examine whether the increase of electroacupuncture(EA) intensity could offer higher analgesic effect ,the difference between responder group and non-responder group , using c-Fos immunohistochemistry method .We also discuss that noxious stimulation to the hindlimb resulted in a massive increase in the expression of fos in neurons in the dorsal horn of the lumbar spinal cord . Fos was recently emphasized as a major tool in the study of mechanism of acupuncture analgesia .The fos expression in central nervous system after EA stimulation represent the integration and modification of neuronal activity noxious and analgesic input . Though the specificity , stability , and repeatability , c-Fos expression are superior to those of traditional behavior response . The combination of c-Fos expression to EA stimulation will result in more information . Method：All Wistar rats were anesthetized with 1% of halothane in oxygen Experimental rat received .EA stimulation of 2Hz or 100Hz at Zusali（S36）in the right hind limb for 30mins . Tail flick latency of all rats was measured at 10 min interval from 30mins before EA to 30mins after EA .Rat in morphine group received intraperitoneal morphine injection .Maximal possible effect and c-Fos amount of lumbar spinal cord （L1-L5）were calculated for statistical analysis . Result：2Hz or 100Hz EA stimulation in tail flick latency time show prolongation . Responder group（RG）was appeared significant analgesic effect in 2Hz or 100Hz EA stimulation（p<0.001），non-responder group（NRG）was too .In c-Fos amount aspect ， It had not significant difference both RG and NRG of using 2Hz EA stimulation . But 100Hz EA stimulation at deep layer of L3、L4 appear significant difference in stimulation side （right）and non-stimulation side（left）（p<0.05）.Superficial layer and Nucleus Propius layer had not significant finding. Conclusion：Our result proved low and high frequency peripheral EA stimulation prolonged the tail flick latency .100Hz EA stimulation produce stronger analgesic effect，and also produce noxious stimulation .In c-Fos distribution , we could found some relationships in neuroanatomy field.