Study on biomarkers of oil smoke exposure

• Main Authors: Huang Kuo Hao, 黃國豪
• Other Authors: H. Lee
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/03278290924431583397

碩士 === 中山醫學院 === 毒理學研究所 === 87 === Previous epidemiological data indicated that the incidence of lung cancer in Chinese female may be related with the exposure of oil smokes from cooking. Our previous studies showed that at least three carcinogenic compounds (benzo[a]prene, MeIQx, and 1,-dinitropyrene) have found in oil smokes from frying fish. These three compounds have been demonstrated to be lung carcinogens in rodents. In addition, 8-hydroxydeoxyguanosine (8-OH-dG) was formed when the lung adenocarcinoma CL-3 cells was treated with oil smokes. Those results suggest that oil smokes can induce two kinds of DNA damages, such as bulky DNA adducts and oxidative stress. Previous study has found that oil smokes contain 1,3-butadiene, which is a carcinogen. Therefore, we tried to establish the biomarkers of oil smoke exposure in this study, mRNA expressions of CYP1A1, CYP2E1, and OGG1 were chosen as candidates ,which involved in the metabolisms of carcinogens in oil smokes and repair pathway of 8-OH-dG. 238 blood samples of healthy persons were collected including 94 cooks and 43 housewives as oil smoke exposure group, and 101 students as controls. The total RNA was extracted from lymphocytes and RT-PCR was used to evaluate the mRNA expression of these genes. Our data indicated that CYP1A1, CYP2E1, and OGG1 mRNA expression were respectively associated with the exposure of oil smokes (OR = 26.93, 95%CI = 7.14-101.54 for CYP1A1; OR = 10.72, 95%CI = 3.43-33.56 for CYP2E1; OR = 3.69, 95%CI = 1.48-9.18 for OGG1). In addition, OGG1 mRNA expression to oil smoke exposure may be more sensitive than two other genes, because OGG1 mRNA expression was relatively detectable in low exposure oil smokes, such as housewives (OR = 4.34, 95%CI = 1.17-16.07). Smoking and drinking habits did not influence the expressions of these three genes. However, the OR values of these three genes in subjects with drinking habits were higher than those without drinking. In conclusion, the three genes, CYP1A1, CYP2E1, and OGG1 mRNA expression detected by RT-PCR may be acted as suitable biomarkers of oil smoke exposure. This result may be helpful to monitoring human health hazard induced by exposure of oil smokes.