| Summary: | 碩士 === 高雄醫學院 === 天然藥物研究所 === 87 === An EtOH extract of the stems of Euonymus laxiflorus Champ. (Celastraceae) and Celastrus hindsii Benth. exhibited cytotoxicity against caroma in vitro. From the stems of Euonymus laxiflorus, we isolated and characterizated of twenty one compounds, including of four sesquiterpene alkaloids, ebenifoline E-II (1), mayteine (2), euojaponine C (3), emarginatine-E (4); sevene triterpenoids, 3-hydroxyolean-12-en-22, 29-g-lactone (5), laxiflone-A (6), 3, 11-dioxo-b-amyrene (7), 3b, 22a-dihydroxyolean-12-en-29-oic acid (8), 28, 29-dihydroxyfriedelan-3-one (9), 29-hydroxy-3-oxo-D:A-friedooleanan-28-oic acid (10) and putranjivadione (11); six benzenoids, 4'-hydroxy-3'-methoxy-trans-cinnamaldehyde (12), p-Hydroxybenzoic acid (13), vanillin (14), isovanillin (15), isovanillic acid (16) and syringic acid (17); four steroids, b-sitosterol-D-glucoside (18), stigmasterol-D-glucoside (19), b-sitosterol (20) and stigmasterol (21). Two cyclohexanes, crotepoxide (22) and (-)-(1R, 2R, 4R, 5S, 6R, 7R)-4-benzoyloxymethyl-3, 8-dioxatricyclo [5.1.0.02,4] octane-5, 6-diol-6-acetate (23); two neolignans, futoenone (24) and (±)-(2a, 4a, 5ab)-4-(4-hydroxy-3-methoxyphenyl)-2, 5a-methano-7-methoxy-5-methyl-8H-2, 3, 4, 5-tetrahydro-1-ben-zoxepin-8-one (25) were isolated from the leaves of Euonymus laxiflorus. Two sesquiterpene polyol esters, celahin-D (26) and celahin-A (27); two triterpenes, loranthol (28) and friedelinol (29) were isolated from the stems of Celastrus hindsii.
Among them, 6 and 26 are new compounds. 22-24 and 25 are isolated for the first time from Family Celastreaceae. The structures of these compounds were determined on the basis of spectral analysis.
Biological evaluation showed that 22, 24 demonstrated cytotoxicity against Human colon carcinoma (colon-205, ED50 < 1.0 mg/ml).
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