Studies on Shoot tip Culture of Indian Jujube (Zizyphus mauritiana Lam.) in Vitro.

• Main Authors: Ju-Yu Yeh, 葉如毓
• Other Authors: Yau-Shiang Yang
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/17854129810533363750

碩士 === 國立中興大學 === 園藝學系 === 87 === Shoot tip culture of the Kaung Laang No.1 Indian jujube (Zizyphus mauritiana Lam.) was studied, optimal micropropagation, shoot rooting and acclimatization were investigated. Shoot tip were harvested about 5 cm in lengh from actively field grown trees and shoot apices were excised 0.5 mm for intital culture. The best grown were achieved when shoot apices culture on solid medium with 1/2 MS strength of Murashige and Skoog (MS) cotaining BA 0.1 mg/l and IBA 0.5 mg/l , sucrose 30 g/l and agar 8g/l and inbubated in darkness for 1 days, then incubated in lightness for 29 days. These explants were transferred to the same component solid medium for futher growth . Multiple shoots were induced by 1/2 MS mediun cotaining sucrose 30 g/l and agar 8 g/l plus kinetin 0.05 mg/l and IBA 0.05 mg/l for 30 days. For rooting, the shoots grew above 10 mm in height, were placed on 1/2 MS medium IBA 2.5 mg/l, sucrose 30 g/l and agar 6 g/l and incubated in darkness for 7days, then transplanted to an auxin-free 1/2 MS medium for 23 days. Rooted plantltes were gradually acclimatized during two weels, and planted in plastic baskets filled with peatmoss and perlite. The plantets became healthy and developed vigorus roots after five months.