The Serum Biochemical Profile of Formosan puppies

• Main Authors: Lai Fu-Chien, 賴富健
• Other Authors: 馮翰鵬
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/79848972932064255460

碩士 === 國立中興大學 === 獸醫學系 === 87 === Serum biochemistry is used as an important indicator not only for disease diagnosis, but also for physiological and pharmacological examinations in animals. At present, the principle biochemical values of canines are based on adult samples. However, biochemical variations in puppies during their growing process is seldem reported. Eleven newborn Formosan puppies were used as the experimental group and six adults with an average age of 2.4 years old as the control group. The variations of serum biochemical profile in 2 groups were continuously observed. Collections of puppy serum samples were performed at birth and 24 hours after parturition, and then once a week for 28 weeks. The collection time of blood samples was identical in adults. The serum biochemical components, including alanine aminotransferase( ALT ), alkaline phosphatase( ALP ), aspartate aminotransferse( AST ), blood urea nitrogen( BUN ), calcium( Ca ), creatinine, g-glutamyl transpeptidase( GGT ), glucose, inorganic phosphate( IP ), lactate dehydrogenase( LDH ), total bilirubin( T.B. ), and total protein( TP ) were examined. All samples were analyzed by a Ciba-CorningO 550 Express Plus clinical biochemical analyze, and the analysis of serum protein and alkaline phosphatase isoenzymes were performed by a Helena Rep electrophoretic analyzer. Upon intake of colostrum, all values of the serum biochemical indices, except creatinine, were increased in the neonatal puppies( 1-day old ). In the growing puppies, levels of Ca、CK、IP、bone ALP( BALP ) and unknown ALP( UALP ) gradually decreased while TP, Alb, b2- and g-globulins gradually increased with age. Although the serum biochemical indices of the puppies were different from the adult samples, they were still in the normal range. Striking variations occurred at two weeks postpartum, but gradually stabilized approaching the adult index. By 28 weeks, the puppy indices appeared no different from that of adults, except ALP, Ca, CK, creatinine, IP, TP, a1-, b1-, b2-, g-globulin, liver ALP( LALP ), BALP and UALP. These results can clearly demonstrate that adult canines are much more ideal than puppies to serve as experimental subjects for physiological examinations.