Development of Chip-based Electrophoresis System with Electrochemical Detection

• Main Authors: Hung Chang Fan, 范弘昌
• Other Authors: Hsien-Chang Chang
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/17983579341107837731

碩士 === 國立成功大學 === 醫學工程研究所 === 87 === Capillary electrophoresis (CE) has become a firmly accepted separation and analysis technique suitable for complex mixtures owing to its ability to provide extremely high separation efficiencies in relatively short time and to do so with small sample volume in low concentration, and to be automatized. There are successively some commercial analyzers available in this decade. Not only the detection systems based on UV/Vis absorption, fluorescence, mass spectrometric method, but also the electrochemical (EC) method including conductive potential or oxidation-reduction current measurements has been published from 1980. This EC-CE system has be proved to possess potential ability for the detection of phenols, neurotransmitters, amino acid, peptides, saccharides. Attractive features of amperometric detection with off-column type designed several microelectrodes at the end of the capillary was the main study, however, the interference caused from the potential field of CE is still a difficult problem. Indeed, many of opinions have been expressed that some short-circuiting techniques, such as cracking the capillary interrupted in front of the working electrode, are requirements for effective EC detection. Among these, the 〝grounded〞detection cells was proposed to reduce the amount of the separation current passing through detection zone, but construction of such cells is difficult. Therefore, we, that having many study experiences in electrochemical sensors, submit this sub-project entitled “Development of Micro-Chip Electrophoresis System Based on Electrochemical Detection” combined with the other three professors specializing in the microfabrication, capillary electrophoresis, and theoretical analysis in fluid. First, the drug compound of Rhodamine B will be used to compare the examined effect based on optical and electrochemical methods. Subsequently, these compounds of FMA, FCA, FDCA and FA having the approximately similar molecular weight but with different electroactive properties used to estimate the determining effect of the micro-electrochemical system. And then neurotransmitter compound of catecholamine mixed compound will be tested for the advanced clinical application.