Effect of Glucose Concentrations on Growth Rate, Energy State and Metabolism of Chinese Hamster Ovary Cell

• Main Authors: Chein-Long Chen, 陳建龍
• Other Authors: Ching-Ping Tseng
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/95526962072205206075

碩士 === 國立交通大學 === 生物科技研究所 === 87 === A variety of Chinese hamster ovary (CHO) cell lines are widely used in the production of therapeutically important proteins. Glucose metabolism in some mammalian cell lines has been investigated on correlation of specific utilization and production rates. In this study, we studied the effect of glucose concentrations on CHO-K1 cell growth, energy state and metabolism. The results showed that the growth rate of Chinese hamster ovary cells was inhibited by high glucose concentration. The cell specific growth rate decreased from 1.3/day to 0.9/day while glucose concentration increased from 1 g/L to 10 g/L. The glucose consumption rate and lactate production rate increased two folds and 40% respectively under high glucose concentration, whereas ammonium production rate was remained constantly. In addition, the intracellular ATP concentration was slightly increased, and ATP/ADP ratio was elevated 40% at high glucose concentration. The intracellular cyclic AMP concentration was 4.5 folds at high glucose concentration of 10 g/L. Hexokinase was the rate-limiting enzyme of glycolytic enzymes and the specific activity of isocitrate dehydrogenase decreased 50% at high glucose concentration. These results demonstrated that CHO cells metabolism and cell growth are controlled by different glucose concentrations in the medium.