A Feasibility Study of Ozone Depletion Free Refrigerant R134a as Mobil Phase Applied in High-Speed Countercurrent Chromatography

• Main Authors: Ya ling Tsai, 蔡雅玲
• Other Authors: Tiing Yu
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/27216674149986236630

碩士 === 國立交通大學 === 應用化學系 === 87 === Since Y. Ito proposed the modern apparatus and techniques for countercurrent chromatography (CCC), many new designs have been developed. Due to the lengthy separation time for traditional CCC, high-speed countercurrent chromatography (HSCCC) comes to the rescue. HSCCC has been proved to be more efficient than the traditional CCC, and has been applied in preparative separations in natural products, biological molecules and pharmaceutical products, etc. Selection of solvent system plays one of the major roles for successful separations. Compound R-134a has been synthesized to replace the traditional coolants, such as chlorofluorocarbons that destroy ozone layer in xx. R-134a is in liquid state when pressurized over 7 bar, and becomes gas under atmosphere pressure. When it is employed as preparative chromatography or extraction fluid, the collected analyte fractions require no further concentration after separation. We examined the feasibility of using R-134a to replace the traditional liquid as the mobile phase in this study. Temperature, pressure and stationary-phase composition effects on separation were investigated. A test separation of several xx standards using this system was also demonstrated.