Sugar Metabolism by Radio Resistant Deinococcus radiodurans

• Main Authors: Huang, Wei-Ball, 黃威球
• Other Authors: Liu, Jong-Kang
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/22799779368596935325

碩士 === 國立中山大學 === 生物科學研究所 === 87 === Deinococcus radiodurans is extemely resistant to ionizing and ultra-violet (UV) irradiation. The addition of Mn(II) (2.5uM) into the stationary-phase clutures would trigger a new round of cell division (Mn-CD. effect), and the radioresistance of D. radiodurans also decreased. The new Mn-CD cells performed differently on sugar metabolism. We found that D. radiodurans can ulitize glucose, fructose, but not galactose. The addition of Mn(II) into the cell culture, a rapid uptake of glucose and fructose but not galactose occured. Further studies using resting cells of D. radiodurans, we found that all sugars tested were not incorporated by the cells even in the presence of Mn(II). We also found the cells had a higher C/N radio in the presence of Mn(II). The addition of glucose (0.1%) and fructose (0.1%) respectively into the growing or resting cell cultures, the UV resistant of D. radiodurans was enhanced. But the addition of galactose (0.1%) to the same culture, the UV resistance was not affected.