Evaluation of the Biological Substance in both Hot Water Extracts and Enzyme Hydrolysates of Muscle from Freshwater Clam,Corbicula fluminea.

• Main Authors: Kung-Kou Feng, 馮貢國
• Other Authors: Zwe-Ling Kong
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/09903907932085323909

碩士 === 國立海洋大學 === 食品科學系 === 87 === The biological substance of hot water extract from freshwater clam (Corbicula fluminea) were shown significantly enhanced the proliferation toward HB4C5. Extracts was purified by stepwise elution on a HyperD and a Prepacked Q column in 20 mMTris-HCl to 20 mMTris 1M NaCl (pH7.9). Successively four fractions called DHN, DHB1, DHB2, DHB3 were separated by Hyper D media. The active ingredient showed on the bound protein fraction DHB1 toward HB4C5. Using the same method purified the DHB1fraction by Prepacked Q column found active ingredient on bound protein fraction QHB2. The QHB2 fraction shown both growth promoting and immune stimulating more effective than Extracts and DHB1 toward HB4C5. Hydrolysate of muscle from freshwater clam shown efficiently on proliferation toward HB4C5 by protease enzyme. The optimal time and enzyme amount were 2 hrs and 2.36% under clam muscle 100g. Subsequent study on efficiency of Hydrolysate by different enzyme, which were Alcalase, Amyloglucosidase, amylase, Flavourzyme, Neutrase, Protamex,and protease. Hydrolysate were hydrolyzed by Protamex and protease show cell proliferation, respectively at the optimal time and enzyme amount were 3 hrs and 8.88% under clam muscle 400g. Meanwhile hydrolysate by protease stimulated IgM production approximately 2-3 fold by UF (YM100 、YM30).