| Summary: | 碩士 === 國立臺灣大學 === 醫事技術學研究所 === 87 === Fungal infections have become a growing threat during the past decade. Frequent and prolonged exposure of immunocompromised patient to a variety of environmental conditions has resulted in the emergence of newly identified fungal pathogens and the reemergence of previously uncommon fungal diseases. Nosocomial infections due to opportunistic fungi are an important cause of morbidity and mortality among hospitalized patients.
Strain typing methods, such as biotyping, serotyping, ribotyping, chromosomal DNA typing, and PCR fingerprinting, have been useful for epidemiological investigation and control of nosocomial infections. The present study demonstrates phenotypic and genotypic characteristics of 11 strains of Exophiala jeanselmei from 9 patients and 20 strains of Penicillium marneffei from 12 patients.
The results of carbohydrate assimilation, antifungl susceptibility, cellular fatty acid analysis and random amplified polymorphic DNA patterns indicate two distinct types of E. jeanselmei and suggest that peudo-outbreak of E. jeanselmei might occur in NTU hospital.
The isolates of P. marneffei were divided into two DNA types according to their restriction fragment length polymorphism. Analysis of random amplified polymorphic DNA patterns obtained by two arbitrary primers showed eight genotypes of P. marneffei. Antifungal susceptibility test and cluster analysis of cellular fatty acid revealed some discrepancies between phenotypic and genotypic methods.
These data suggest that both antifungal susceptibility test and RAPD analysis can be use to investigate the epidemiology of the opportunistic or rare fungal pathogens.
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