Study of Urease-Immobilized Hollow Fiber in Hemodialysis Application

• Main Authors: Wen-Jieh Wu, 吳文杰
• Other Authors: Ming-Chien Yang
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/96691085590324531757

碩士 === 國立臺灣科技大學 === 纖維及高分子工程研究所 === 87 === The performance of the decomposition of urea using urease-immobilized membrane was investigated . Urease was immobilized with glutaraldehyde onto hydrolyzed polyacrylonitrile hollow fibers after graftihg with hexadiamin. The conditions for preparation and the catalyzing effect of the membrane were studied. The surface density of the carboxyl increased with increasing hydrolyzing temperature ,time and concentration of NaOH. The degree of fixation of urease increased with the glutaraldehyde concentration. However, the activity of the immobilized urease was the highest when the glutaraldehyde concentration was 5%. The activity of immobilized urease decreased with increasing pH. At 40℃, immobilized urease had the highest activity. When the temperature was increased, the activity decreased. When the module was reused, the immobilized urease retained highest activity when immobilized with higher glutaraldehyde concentration. The clearance of urea of the module increased with time. When at 30℃and pH 6 the module had highest clearance. In a extracorporeal test, the urease immobilized module can reduce more urea than an ordinary module. The hemodialysis performance of the resulting urease-immobilized hollow fiber, including the hydrolysis and the clearance of urea, are investigated. Show the activity of the immobilized urease.