Transport Pathways of High-Density Lipoproteins Across Arterial Endothelium in Rats
碩士 === 東海大學 === 生物學系 === 87 === The transport pathways of high density lipoprotein (HDL) across the endothelium of artery were studied in high cholesterol diet-fed rats and chow-died controls. Rat tissues were analyed by perfusing in situ rat HDL labeled with collidal gold. Rat high-densi...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
1999
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| Online Access: | http://ndltd.ncl.edu.tw/handle/52391647676257689210 |
| Summary: | 碩士 === 東海大學 === 生物學系 === 87 === The transport pathways of high density lipoprotein (HDL) across the endothelium of artery were studied in high cholesterol diet-fed rats and chow-died controls. Rat tissues were analyed by perfusing in situ rat HDL labeled with collidal gold. Rat high-density lipoprotein was isolated from freshly pooled rat plasma by sequential ultracentrifugation. The KDL-gold conjugates were injected into artery and the tissue were processed for electron microscopy. The results indicated that HDL-gold particles were observed in the plasmalemmal vesicles attached to the luminal or abluminal surface as well as in the subendothelial space. HDL-gold particles were also observed in the open junctions at the branched region of the arteries. However, the HDL-gold particles are not revealed in the fatty streak after 12 months of high cholesterol diet feeding. The results indicated that the major visible routes for transport of HDL across the endothelium is plasmalemmal vesicles. The open junctions between endothelial cells at the branched region of the arteries also may play an important role in the transendothelial transport.
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