The Analyses of Ginsenosides from Ginseng Drug Preparations and Ganoderic Acid from Ganoderma lucidum by High-performance Liquid Chromatography and Capillary Electrophoresis

• Main Authors: Shu-Hui Wu, 吳淑惠
• Other Authors: Wen-Fa Sye
• Format: Others
• Language: zh-TW
• Published: 1999
• Online Access: http://ndltd.ncl.edu.tw/handle/94833083352007605679

碩士 === 淡江大學 === 化學學系 === 87 === Title of Thesis: The Analyses of Ginsenosides from Ginseng Drug Preparations and Ganoderic Total Pages:139 Acid from Ganoderma lucidum by High-performance Liquid Chromatography and Capillary Electrophoresis Key Word: Sep-Pak C18，Ginseng，Ginseng drug preparations，Ganoderma lucidum，High-performance liquid chromatography，Capillary electrophoresis Name of Institute:Graduate Institute of Chemistry, Tamkang University Graduate Date: January, 1999 Degree Conferred: Master Name of Student: Shu-Hui Wu Advisor: Dr. Wen-Fa Sye 吳淑惠 薛文發 Abstract: Ginseng and Ganoderma lucidum play important roles in Chinese medicine. Ginseng has widely been used in traditional Chinese medicine. There are many reports of pharmacological and chemical studies on ginseng saponins. The fungus Ganoderma lucidum has been used in traditional oriental medicine for the treatment of various diseases and as an antitumor agent. The study of the effective components contained in Ginseng and Ganoderma lucidum helps a lot in their medical applications. Common preliminary procedures for the isolation of ginsenosides from ginseng drug preparations and ganoderic acids from Ganoderma lucidum involve solvent extraction or extraction by adsorbents. Such methods demand a great deal of working time because of the phase separation and experimental procedures. In this thesis, solid phase extractions of Sep-Pak C18 Cartridges were used to the extractions of ginsenosides and ganoderic acids. An extraction using Sep-Pak C18 Cartridges is a simple, rapid and reproducible methods. Sep-Pak C18 Cartridges provide a superior technique for the sample cleanup and concentration. After solid phase extractions, the extracts were analyzed by high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis is becoming increasingly recognized as an important analytical separation technique as a result of its speed, high reproducibility and high efficieny. As a result, ginsenosides Re/Rg1、Rb1、Rc、Rb2、Rd from ginseng drug preparations and ganoderic acids A、B、C、D from Ganoderma lucidum can be effectively separated by high-performance liquid chromatography. The use of micellar electrokinetic capillary chramatography can also partly separate ginsenosides in ten minutes. As for the separation of ganoderic acids, we need further research work.